Assessment of Enzyme Activity and Functional Microbial Diversity in Coastal and Desert Soil Ecosystems of Gujarat
Journal Title: Journal of Enzymology and Metabolism - Year 2016, Vol 2, Issue 1
Abstract
All living beings of various trophic levels in ecosystem depend on the soil as a source of nutrients and depend on soil organisms to release and recycle key nutrient elements by decomposing organic residues. Microbiota of soil plays critical role in the maintenance of soil health and quality by secreting important enzymes, which are capable of function even after being released by the cell. Comparing various types of soils, salinity is profoundly observed in coastal and desert region. Study of such type of soil may give insights for understanding the variations in microbial community and effect of various abiotic factors like, salt concentrations on the same. Studying diversity at the ecosystem level is important to understand range of processes and complexity of interactions. Polyphasic approach of studying microbial community by C source utilization profiling and soil enzyme activity measurement was employed in this study to compare desert and costal ecosystems. Functional diversity was studied by CLPP method using Ecoplate®. Six different soil enzyme activities were investigated. In some samples protease, urease and L-asparaginase activity were not detected at all. β glucosidase and L-asparaginase showed significant positive correlation with all functional diversity indices, most of the microbial groups and temperature while negatively correlated with moisture and pH respectively. Alkaline phosphatase activity negatively correlated with temperature. PCA analysis based on enzyme activity showed that samples were grouped together geographically or according to the source of origin.
Authors and Affiliations
Nasreen S Munshi
Keywords
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