Assessment of Genetic diversity of Gymnema sylvestre (Retz.) R.Br. from Western Ghats and Eastern India, India
Journal Title: Journal of Biodiversity and Environmental Sciences (JBES) - Year 2016, Vol 9, Issue 1
Abstract
To device management strategies for conservation of plant, the complete knowledge of genetic variation within and among populations of plant species is essential. Inter simple sequence repeat and Random amplified polymorphic DNA markers were used to study the genetic diversity and population genetic structure in 117 accessions of 11 populations of Gymnema sylvestre, an important anti diabetic plant from Western Ghats and Eastern India of India. A total of 7 ISSR and 6 RAPD selected primers produced 3474 and 4104 discernible bands respectively, with 100% polymorphism. The Nei’s gene diversity (h) was found to be 0.27 and 0.26 at the species level based on ISSR and RAPD markers respectively indicating high genetic diversity. The Shannon’s index (I) was estimated to be 0.42 and 0.41 at the species level based on ISSR and RAPD markers respectively. The analysis of molecular variance showed that the genetic variation was found equally within populations (ISSR = 55% and RAPD = 51%) and among populations (ISSR = 45% and RAPD = 49%) both. In addition, Nei’s differentiation coefficient (GST) was found to be high (ISSR = 0.70 and RAPD = 0.60) and the gene flow (Nm) was low (ISSR = 0.21 and RAPD = 0.33), confirming the high population genetic differentiation. There was high genetic differentiation and the low gene flow in this species of plant, which suggests that conservation should emphasis on preserving the populations and which will also help the concerned authorities in designing strategies on the conservation of G. sylvestre.
Authors and Affiliations
Poonam K. Rathore, Samreen Madihalli, Satisha Hegde, Harsha V. Hegde, Rasika M. Bhagwat, Vidya S. Gupta, Sanjiva D. Kholkute, Timir baran Jha, Subarna Roy
Keywords
Related Articles
- EP ID EP38856
- DOI -
- Views 192
- Downloads 0
Ethno-botanical survey of some anticancer medicinal plants from Garhwal Himalaya (Uttarakhand) India
Ethno-botanical survey of the plants used in the management of cancer was carried out in Garhwal Himalaya, Uttarakhand, India. Herbalists, herb sellers and traditionalists living within the study area were interviewed b...
Diversity of bananas (Musa spp.) in Madura Island, East Java: exploration and inventory
Banana exploration study has been conducted in Madura Island covering areas of Bangkalan, Sampang, Pamekasan and Sumenep Districts. This paper presents the results of survey, inventory and diversity of bananas in Madur...
Single drop scanometry, Determination of cyanide in water in the various color spaces.
In this study, the application of new and simple single drop scanometric method was described for determination of cyanide as alternative for visible spectrophotometric method. Cyanide ion reacts with the methyl violet a...
Historical overview of customs crimes and ways of addressing them
Iran's government suspended the organization, was established to monitor economic activity and trade; but the arrival of great volume of contraband into the country and the inadequacy of the justice system's ability to...
Abundance and population profile of Helicostyla daphnis (Stylommatophora: Bradybaenidae), an endemic snail of Cebu, Philippines
Helicostyla daphnis is a tree snail endemic to Cebu, Philippines. The study aimed to survey and compare the abundance and population profile (by age category) of Helicostyla daphnis in North Cebu and South Cebu in rela...