Assessment of In Vitro Antigenotoxic Effect of Nigella Sativa Oil
Journal Title: Turkish Journal of Pharmaceutical Sciences - Year 2020, Vol 17, Issue 1
Abstract
Objectives: Cyclophosphamide (CP) is an alkylating agent widely used as an antineoplastic and immunosuppressive agent. The genotoxicity of CP has been studied in a variety of in vivo and in vitro systems and is routinely used as a positive control in genotoxicity tests. Traditional medicine Nigella sativa L., (N. sativa), Ranunculaceae family, especially in the Eastern Mediterranean countries, especially in many countries, and is widely used in many countries as a spice and folk medicine since the time of Dioscorides used as a plant. In this study, it was aimed to show the protective effects of N. sativa oil at different concentrations against the genotoxic effects of CP by micronucleus test. Materials and Methods: For this purpose, healthy cells were treated in vitro with N. sativa oil at concentrations of 1, 5, 10 μg/mL and CP as positive control for 68 hours. The micronuclei were then counted. Results: No significant increase in micronucleus frequency was observed when the application of N. sativa oil at concentrations of 1, 5, 10 μg/mL compared with the negative control. There was a decrease in the number of micronucleus in all three concentrations (1, 5, 10 μg/mL) compared to the CP group in the groups treated with N. sativa oil and CP. Conclusion: It has been shown that N. sativa oil may have protective effects against genotoxicity agents in vitro. But more work is needed to understand the mechanism of the genotoxicity effects of N. sativa oil.
Authors and Affiliations
Murat ZOR, Elçin Latife ASLAN
Keywords
Related Articles
- EP ID EP684738
- DOI 10.4274/tjps.galenos.2020.09471
- Views 173
- Downloads 0
Synthesis and Swelling Behavior of Sodium Alginate/Poly(vinyl alcohol) Hydrogels
Objectives: Hydrogels are macromolecular networks able to absorb and release water/biological fluids in a reverse-phase manner, in response to specific environmental stimuli. Such stimuli-sensitive behavior makes hydroge...
Psychoactive Bath Salts and Neurotoxicity Risk
Synthetic cathinones are new designer drugs that possess hallucinogenic and psychostimulant properties, and are designed to mimic the effects of illegal substances such as cocaine, amphetamines, and 3.4-methylenedioxymet...
In Vitro Antimicrobial and Antioxidant Activity of Biogenically Synthesized Palladium and Platinum Nanoparticles Using Botryococcus braunii
Objectives: The spread of infectious diseases and the increase in drug resistance among microbes has forced researchers to synthesize biologically active nanoparticles. Ecofriendly procedures for the synthesis of nanopar...
Optimization of Immobilized Aldose Reductase Isolated from Bovine Liver
Objectives: Isolation of enzymes and experiments on them require great effort and cost and are time-consuming. Therefore, it is important to extend the usability of the enzymes by immobilizing them. In this study our pur...
An In Vitro Study on the Cytotoxicity and Genotoxicity of Silver Sulfide Quantum Dots Coated with Meso-2,3-dimercaptosuccinic Acid
Objectives: Silver sulfide (Ag2S) quantum dots (QDs) are highly promising nanomaterials in bioimaging systems due to their high activities for both imaging and drug/gene delivery. There is insufficient research on the to...