Assessment of the antioxidant potential of Pergularia daemia (Forsk.) extract in vitro and in vivo experiments on hamster buccal pouch carcinogenesis

Journal Title: Asian Pacific Journal of Tropical Disease - Year 2012, Vol 2, Issue 0

Abstract

Objective: The aim of the present study was to investigate the in vitro antioxidant assays and in vivo non enzymatic antioxidant potential of aerial parts of Pergularia daemia methanolic extract (PDME) on 7, 12-dimethylbenz(a)anthracene (DMBA) induced hamster buccal pouch (HBP) carcinogenesis. Methods: The plant extract was tested for their total phenol and flavonoid content and radical scavenging effect such as 2, 2′-azinobis- (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS● +) radical decolorization assay, DPPH (2, 2-diphenyl, 2-picryl hydrazyl) radical scavenging, nitric oxide radical scavenging, reducing power assays. Results: According to IC50 values obtained, PDME contains more powerful antioxidant compounds than gallic acid. The level of plasma vitamin C and the levels of Vitamin E and GSH in the plasma and erythrocyte lysate were decreased while the buccal tissue levels of vitamin E and GSH were increased in DMBA induced animals. Pretreatment with P.daemia (200 mg/kg bw) to DMBA induced animals significantly (P<0.05) increased the levels of plasma and erythrocyte vitamin C, vitamin E and GSH where as the levels of vitamin E and GSH in the buccal tissue were increased compared to DMBA alone induced animals. Conclusions: The PDME exhibited strong antioxidant activity in free radical scavenging assays and hamster buccal pouch carcinogenesis.

Authors and Affiliations

Keywords

Related Articles

Leptospirosis presenting as acute encephalitis syndrome (AES) in Assam, India

Objective: To establish leptospirosis as a new aetiology of the patients presenting acute encephalitis syndrome (AES). Methods: Japanese encephalitis, West Nile, Dengue and Chikungunya negative samples were tested by IgM...

Medicinal plants and secondary metabolites for diabetes mellitus control

Diabetes mellitus is one of the most common and complex problems of modern societies which has caused many economic and social problems. Because diabetes has no definite treatment, the use of traditional medicine seems t...

Antimicrobial activity and composition of the volatiles of Cinnamomum tamala Nees. and Murraya koenigii (L.) Spreng. from Uttarakhand (India)

Objective: To examine the composition of Cinnamomum tamala and Murraya koenigii essential oils and their antimicrobial activities against nine microbial strains. Methods: Essential oils were obtained by hydrodistillation...

Burden estimation of dengue at National Public Health Laboratory, Kathmandu

Objective: To determine the burden of dengue in the patients visiting National Public Health Laboratory, Kathmandu. Methods: A cross sectional study was carried out at National Public Health Laboratory, Kathmandu from Ma...

Lymphocystis in Indian oil sardine, Sardinella longiceps (Valenciennes 1847)

Objective: To analyse the prevalence and pathology of lymphocystis disease in Sardinella longiceps of three different landing centers in Tamil Nadu of southeast coast of India for a period of three years from January 201...

Download PDF file
  • EP ID EP233736
  • DOI 10.1016/S2222-1808(12)60212-6
  • Views 94
  • Downloads 0

How To Cite

(2012). Assessment of the antioxidant potential of Pergularia daemia (Forsk.) extract in vitro and in vivo experiments on hamster buccal pouch carcinogenesis. Asian Pacific Journal of Tropical Disease, 2(0), 509-516. https://www.europub.co.uk/articles/-A-233736