Assessment of Three Anesthetics Used for Pulmonary Pharmacological Studies in Rats
Journal Title: Journal of Applied Veterinary Sciences - Year 2022, Vol 7, Issue 2
Abstract
Experiments on pulmonary pharmacology are often performed in laboratory animals. Investigators collect organs, samples, or record modifications in the respiratory system during anaesthesia, which might significantly influence the data attained. Indeed, it is recommended to cautiously select the anaesthetic adapted for lung studies. This experiment aimed to evaluate three selected anaesthetics usually used in laboratory animal experiments, namely, (Zolite-rumpon (Z+R) mixture, halothane (HAL), and urethane (URE)) in rats. Rats were divided into four groups, control group, kept without treatment; Zolitel and Rumpon (Z+R) at a dose of 5 and 30 mg/kg body weight intraperitoneally, respectively; HAL group (5% with oxygen supply), and URE (1.5 mg/kg body weight) by inhalation. Blood samples were collected to determine the pH of the blood, bicarbonate (HCO3-), carbon dioxide partial pressure (pCO2), oxygen partial pressure (pO2), alveolar oxygen (A), saturated oxygen (O2 Sat), magnesium (Mg2+), calcium (Ca2+), sodium (Na+), potassium (K+), and chloride (Cl−) ions, haematocrit (Hct), and haemoglobin (Hb). The result indicates that the Z+R mixture causes slight to no differences in the measured data. At the same time, HAL and URE greatly reduced the blood pH and significantly disrupted the values of HCO3-, pCO2, pO2, A, and O2 Sat. Together, HAL and URE induced metabolic acidosis and respiratory depression. Hence, the Z+R mix is suitable for the three anaesthetics pulmonary pharmacology experiments.
Authors and Affiliations
Gareeballah Osman Adam
Impact of Eruca sativa Leaves Aqueous Extract on Liver Function, Immunity Profile and Behavioural Responses of Healthy and Thioacetamide Intoxicated Male Albino Mice
The current experiment was intended to investigate the hepatoprotective, immunostimulant activity, behavioural and anxiety responses of mice supplemented with Eruca sativa aqueous extract (ESAE) at a dose of 1% in drinki...
Haemato-biochemical Response to Kirschner Pin and Improvised Chrome Vanadium Long Screw Used for the Stabilization of Femoral Fracture in Goats
The stainless-steel orthopaedic implants used for the management of various types of fracture internal fixations are very expensive; this makes their utilization in food animals relatively noneconomical. There is a need...
Treatment of Mucopurulent Endometritis in a Rhesus Monkey: A Case Study
This report describes the treatment of Mucopurulent Endometritis in a Rhesus monkey. The involved she-monkey was three years old and weighing five kg body weight lived as a home animal, brought by the owner to the clinic...
Effects of Pesticide Residues in Animal By-products Relating to Public Health
Pesticides are used on a large scale and have become unavoidable in the modern agricultural economy. Tones of them are used on a daily basis for a variety of functions, which are mostly used for agricultural activities....
Isolation and characterization of stx1 and stx2 toxin-producing Escherichia coli in neonatal lambs with diarrhea in Nineveh governorate, Iraq
Escherichia coli causes many health problems in lambs, the most prominent of which is diarrhea, the study aimed to detect the shiga toxins genes in E.coli isolated from the suckling lambs affected with diarrhea in Nineve...