Assessment of Three Anesthetics Used for Pulmonary Pharmacological Studies in Rats
Journal Title: Journal of Applied Veterinary Sciences - Year 2022, Vol 7, Issue 2
Abstract
Experiments on pulmonary pharmacology are often performed in laboratory animals. Investigators collect organs, samples, or record modifications in the respiratory system during anaesthesia, which might significantly influence the data attained. Indeed, it is recommended to cautiously select the anaesthetic adapted for lung studies. This experiment aimed to evaluate three selected anaesthetics usually used in laboratory animal experiments, namely, (Zolite-rumpon (Z+R) mixture, halothane (HAL), and urethane (URE)) in rats. Rats were divided into four groups, control group, kept without treatment; Zolitel and Rumpon (Z+R) at a dose of 5 and 30 mg/kg body weight intraperitoneally, respectively; HAL group (5% with oxygen supply), and URE (1.5 mg/kg body weight) by inhalation. Blood samples were collected to determine the pH of the blood, bicarbonate (HCO3-), carbon dioxide partial pressure (pCO2), oxygen partial pressure (pO2), alveolar oxygen (A), saturated oxygen (O2 Sat), magnesium (Mg2+), calcium (Ca2+), sodium (Na+), potassium (K+), and chloride (Cl−) ions, haematocrit (Hct), and haemoglobin (Hb). The result indicates that the Z+R mixture causes slight to no differences in the measured data. At the same time, HAL and URE greatly reduced the blood pH and significantly disrupted the values of HCO3-, pCO2, pO2, A, and O2 Sat. Together, HAL and URE induced metabolic acidosis and respiratory depression. Hence, the Z+R mix is suitable for the three anaesthetics pulmonary pharmacology experiments.
Authors and Affiliations
Gareeballah Osman Adam
Keywords
Related Articles
- EP ID EP702598
- DOI 10.21608/JAVS.2022.119861.1123
- Views 92
- Downloads 0
Chicken Egg Yolk-IgY: Passive Immunization Promising Targeted Therapy of COVID-19 Pandemic
The world is threatened by the lethal effects of Coronaviruses (CoVs) that associated with major respiratory disorders. The CoVs are large, enveloped, positive-stranded RNA and therefore, it is considered a complicated v...
Serological Detection of Newcastle Disease Virus Antibody in Vaccinated and Non-Vaccinated Indigenous Chickens and Guinea Fowls in Atacora and Donga, Northern Benin
Poultry farming is crucial for the livelihoods of small-scale producers in the Northen Benin, yet the industry faces challenges from diseases like ND, which threaten both poultry health and economic stability. This study...
Hemagglutinin Gene Diversity of Canine Distemper Virus from Clinical Samples in Brazil
Canine distemper virus (CDV) affecting both domestic dogs and wild animals, resulting in high rates of morbimortality. The viral genome encodes six different proteins where the Hemagglutinin is the most variable, being t...
The Effects of Sub-lethal Concentrations of Sodium Fluoride on the Number and Diameters of Ovarian Follicles
Fluoride was the first compound utilized in the fluoridation of drinking water, and still used today t0 for prevent dental caries, sodium fluoride exerts it toxic effects on a different organs . Ovarian follicle consider...
Seroprevalence of toxoplasma gondii and brucella abortus in dairy animals from the Sudan: special emphasis to their serological co-existence
Toxoplasmosis and brucellosis are the most common zoonotic food borne diseases worldwide. The abortion caused by these agents is one of the major causes of socio-economic losses in human and livestock. We thus conducted...