Assessment Of Total RNA Extraction From Edible Mushroom (Agaricus bisporus) With Three Current Methods
Journal Title: International Journal of Farming and Allied Sciences - Year 2013, Vol 2, Issue 18
Abstract
Nucleic acid extraction is an attractive alternative to labor-intensive manual methods. In this report, a few methods of total RNA Extraction from Edible mushroom (Agaricus bisporus) compared. This methods consist AGPC(Acid Guanidinium-Phenol-Chloroform), The method according by Lithium chloride and RNx total RNA isolation Kit (CinnaGen). Best method selected for the preparation of high quality and intact total RNA from A. bisporus. Based on the Ethidium Bromide straining pattern of 28s and 18s rRNAs, either denaturing (formaldehyde/agarose) or nondenaturing (TAE/agarose) gel electrophoresis could be used to determine the general integrity of the RNA preparation. Analysis by either method revealed sharp rRNA bands indicative of high quality RNA. yield of RNA was acceptable in any three method but RNA quality in AGPC method similar with RNx Total RNA isolation kit (CinnaGen).
Authors and Affiliations
Javad Janpoor, Mohammad Farsi
Keywords
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