Assessment of viability of the nematode eggs ([i]Ascaris[/i], [i]Toxocara[/i], [i]Trichuris[/i]) in sewage sludge with the use of LIVE/DEAD Bacterial Viability Kit
Journal Title: Annals of Agricultural and Environmental Medicine - Year 2014, Vol 21, Issue 1
Abstract
Sewage sludges from wastewater treatment plants may contain live parasite eggs, which can be a source of humans and animals infection. According to the current rules, parasitological examination includes detection of the [i]Ascaris [/i]spp., [i]Trichuris [/i]spp. [i]and Toxocara [/i]spp. eggs and estimation of their viability. The viability assessment based only on the incubation and observation of isolated egg is long and imprecise. The aim of this study was to develop sensitive and less labourintensive methods for assessing viability of [i]Ascaris [/i]spp[i]., Toxocara [/i]spp[i]. and Trichuris [/i]spp[i].[/i] eggs in sewage sludge. For this purpose, LIVE/DEAD Kit was used. Firstly, the possibility of distinguishing between live and dead eggs in water was assessed. Secondly, an appropriate amount of dyeing mixture needed to distinguish the live and dead eggs in the sewage sludge was determined using experimentally enriched samples and naturally contaminated samples of sludge. Eggs were isolated from the samples by own method which was a combination of flotation and sedimentation, preceded by a long mixing. After the last stage of the procedure, sediment containing the eggs of parasites was stained by LIVE/DEAD kit according to the manufacturer instructions, but with the use of different variants of dyes mixture concentration. The investigation showed that live and dead eggs of these three parasites could be differed by this method with the use of proper concentration of dyes. Live eggs were stained in green ([i]Ascaris and Trichuris[/i]) and green-blue ([i]Toxocara[/i]). However, all types of dead eggs were red coloured. The study demonstrated that after some modifications (resulted from the nature of the samples) the LIVE/DEAD kit is useful for assessing the viability of [i]Toxocara[/i], [i]Ascaris[/i] and [i]Trichuris[/i] eggs occurring in the sludge.
Authors and Affiliations
Joanna Dąbrowska, Jolanta Zdybel, Jacek Karamon, Maciej Kochanowski, Krzysztof Stojecki, Tomasz Cencek, Teresa Kłapeć
Keywords
Related Articles
- EP ID EP80733
- DOI -
- Views 134
- Downloads 0
Prevalence of [i]Legionella pneumophila[/i] in water distribution systems in hospitals and public buildings of the Lublin region of eastern Poland
Objective. The aim of this study was to determine the prevalence of [i]L. pneumophila[/i] in water supply systems, hospitals and public buildings in the Lublin region of eastern Poland. Material and methods. The study...
Cytotoxic and Antiviral Compounds from Bryophytes and Inedible Fungi
Over several hundred new compounds have been isolated from the bryophytes and more than 40 new carbon skeletalterpenoids and aromatic compounds found in this class. Most of liverworts elaborate characteristic odiferous,...
Effect of N-(p-ethoxycarbonylphenylmethyl) -p-isopropoxyphenylsuccinimide on the anticonvulsant action of four classical antiepileptic drugs in the mouse maximal electroshock-induced seizure model
[b]Introduction and objective[/b]. The purpose of this study was to determine the effects of N-(p-ethoxycarbonylphenylmethyl)-p-isopropoxyphenylsuccinimide (ECPM-IPPS), a new succinimide derivative, on the protective act...
Assessment of intake of iron and nutrients that affect bioavailability of daily food rations of girls
INTRODUCTION AND AIMIn a human body iron occurs at a level of 3 to 5 g, 60-70 % of which are in hemoglobin, ca. 10% in myoglobin, and ca. 3% are accumulated in enzymes of cellular respiration or enzymes degrading toxic h...
Visual assessment of voice disorders in patients with occupational dysphonia
[b]introduction[/b]. In a group of persons using the voice occupationally, the frequent symptoms are hoarseness, voice fatigability and aphonia. Pathological changes in the larynx may have organic or functional character...