Asymbiotic In vitro Seed Germination and Regeneration of Vanda coerulea Giff. Ex. Lindl., an Endangered Orchid from Northeast India
Journal Title: Journal of Plant Science and Research - Year 2015, Vol 2, Issue 2
Abstract
Blue Vanda (Vanda coerulea Giff. ex. Lindl.) is one of the most beautiful orchids found in the northeastern region of India. Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) listed this species in the appendix 1 as a vulnerable one. In the present study asymbiotic in vitro seed germination and regeneration of V. coerulea via protocorm formation was achieved in MS basal medium. Immature seeds cultured on full strength MS basal medium showed higher rate of germination with subsequent formation of ‘protocorm like bodies’ (PLB) in comparison to half strength MS basal medium. Multiplication of the protocorms and differentiation into shoots was attained in MS basal medium supplemented with combination of benzylamino purine (BA) and kinetin (Kn). Highest number of leaf emergence was observed in MS basal medium supplemented with 22.80 µM indole acetic acid (IAA). Supplementation of basal medium with banana extract (75 mg L-1) resulted in highest number of root induction. The standardized protocol for efficient in vitro germination of immature V. coerulea seeds and their subsequent regeneration can be applied for future mass propagation and ex situ conservation of this endangered orchid.
Authors and Affiliations
Robert Thangjam
Keywords
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