Attenuation of oxidative stress-induced neuronal cell death by Hydnophytum formicarum Jack.
Journal Title: Asian Pacific Journal of Tropical Medicine - Year 2018, Vol 11, Issue 7
Abstract
Objective: To investigate protective effects of Hydnophytum formicarum Jack. (H. formicarum) extracts via regulation of SIRT1-FOXO3a-ADAM10 signaling and antioxidant activity against H2O2-induced neurotoxicity in neuroblastoma SH-SY5Y cells. Methods: Cell viability and apoptosis of neuronal cells pretreated with H. formicarum Jack. extracts under oxidative stress were determined by MTT assay and flow cytometry. The intracellular reactive oxygen species (ROS) was performed using Carboxy-DCFDA assay. Additionally, a profile of protein expressions related to neuroprotection was detected by western blot analysis. Results: The plant extracts (methanol and ethyl acetate) elicited protective effects on the neuronal cell death as performed by the MTT assay and by apoptosis analysis via the activation of BCL-2. Both ethyl acetate and methanol extracts exerted inhibitory effects against H2O2-induced ROS generation in the SH-SY5Y cells. Furthermore, the possible mechanism of neuroprotection of H. formicarum Jack. was observed through its antioxidant properties by maintaining the levels of catalase and SOD2 proteins as well as activating SIRT1-FOXO3a pathway. Importantly, pretreatment of neuronal cells with H. formicarum Jack. significantly recovered the levels of ADAM10 protein compared with the H2O2 treatment alone. Conclusions: The recent findings suggest the protective effects of H. formicarum Jack. plant extracts on attenuating H2O2-induced neurotoxicity in human SH-SY5Y cells.
Authors and Affiliations
kamonrat Phopin
Keywords
Related Articles
- EP ID EP367179
- DOI 10.4103/1995-7645.237185
- Views 101
- Downloads 0
Knowledge, attitudes and practice survey on Zika virus infection among pregnant women in Brunei Darussalam
Objective: To assess the knowledge, attitudes, and practices (KAP) on Zika virus infection among pregnant women in Brunei Darussalam by a cross-sectional survey. Methods: Between February and June 2017, we recruited 234...
Forecasting the number of zoonotic cutaneous leishmaniasis cases in south of Fars province, Iran using seasonal ARIMA time series method
Objective: To predict the trend of cutaneous leishmaniasis and assess the relationship between the disease trend and weather variables in south of Fars province using Seasonal Autoregressive Integrated Moving Average (SA...
Antibiotic resistance characteristic and genes analysis of a marine water sourced Klebsiella pneumoniae
Objective: Both antibiotic resistant bacteria (ARBs) and antibiotic resistant genes (ARGs) are considered as one of the most dangerous environmental pollutants in the 21st century by the WHO. This study aims to understan...
In vitro anticancer activity of polysaccharide extracted from red alga Jania rubens against breast and colon cancer cell lines
Objective: To evaluate the potential role of the polysaccharides of the marine algae as an anticancer agent in vitro against colon cancer cell line (CoCa2) and breast cancer (MCF7) cell lines and to measure lactate dehyd...
High glucose induces myocardial cell injury through increasing reactive oxygen species production
Objective: To study the injury effect and molecular mechanism of high glucose on myocardial cells. Methods: Myocardial cells H9c2 were cultured and divided into the control group treated with DMEM containing 5.5 mmol/L g...