Bacterial Diversity In Çamaltı Saltern, Turkey
Journal Title: Polish Journal of Microbiology - Year 2015, Vol 64, Issue 1
Abstract
A combination of culture-dependent and culture-independent approaches were employed to identify bacterial diversity of Çamaltı solar saltern in Turkey. The bacterial populations of Çamaltı Saltern were analyzed by molecular techniques that included denaturing gradient gel electrophoresis of 16S rRNA gene fragments PCR amplified from DNA extracted from the saltern and 16S rRNA gene library analysis.A total of 42 isolates were identified at the genus/species level and 17 of them were found to be belonging to the [i]Bacteria[/i] domain. All bacterial isolates were phylogenetically related to [i]Halobacillus, Virgibacillus[/i] and [i]Halomonas[/i] genus. A total of 50 clones from 16S rRNA gene library were analyzed by ARDRA and most (85%) of the bacterial clones were related to [i]Salinibacter[/i] genus members of the Bacteroidetes. The sequences of DGGE bands were related to the Uncultured [i]Salinibacter[/i], Uncultured Halophilic Bacterium and [i]Halomonas[/i] sp. This work highlights the halophilic bacterial diversity of Çamaltı marine solar saltern.
Authors and Affiliations
Mehmet Burcin Mutlu, Kıymet Güven
Keywords
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