Bacteriophages specific for DH5α strain of E. Coli from wastewater isolation techniques
Journal Title: Revista MVZ Cordoba - Year 2012, Vol 17, Issue 1
Abstract
Objetive. To develop an isolation technique for DH5α E. coli specific bacteriophages in wastewater. Materials and methods. The 1601 method of the Environmental Protection Agency of the United States of America and an wastewater enterovirus obtaining method were used as a reference. During the development of the protocol, multiple tests were performed using the T4 bacteriophage as positive control and the bacteriophage isolates obtained from wastewater. Results. Plaque-forming units (PFU) were observed; tittering of bacteriophages present in each of the DH5α E. coli cultures was obtained; the relationships between the quantification of PFU in the control and experimental groups, and their corresponding PFU characteristics were determined for each of the experiments. Conclusions. It was possible to establish a microbiological protocol for the isolation of bacteriophages specific for DH5α E. coli.
Authors and Affiliations
Gabriel Gaviria A, Biól| Universidad del Quindío. Facultad Ciencias de la Salud. Centro de Investigaciones Biomédicas. Grupo de Inmunología Molecular (GYMOL). Carrera 15 Calle 12 Norte, Teléfono: +57(6) 7460168. Armenia, Colombia.Correspondencia: jhoncarlos@uniquindio.edu.co, María González de S, M. Sc| Universidad del Quindío. Facultad Ciencias de la Salud. Centro de Investigaciones Biomédicas. Grupo de Inmunología Molecular (GYMOL). Carrera 15 Calle 12 Norte, Teléfono: +57(6) 7460168. Armenia, Colombia., Jhon Castaño O, Ph. D *| Universidad del Quindío. Facultad Ciencias de la Salud. Centro de Investigaciones Biomédicas. Grupo de Inmunología Molecular (GYMOL). Carrera 15 Calle 12 Norte, Teléfono: +57(6) 7460168. Armenia, Colombia.
Keywords
Related Articles
- EP ID EP3235
- DOI -
- Views 408
- Downloads 19
PRODUCTION OF MEAT WITH STEERS CATTLE BY GRAZING ON HYBRID MULATO PASTURE AND Brachiaria decumbens IN THE SINU VALLEY
In Turipaná research Center of the Colombian Corporation of Agriculture Investigation (Corpoica) a study was carried out with the objective of evaluating the establishment phase, some agronomic variables (plants by squar...
Productive performance of hair lambs, fed with fresh lemon pulp as an energy source
Objective. Evaluate the effect of the inclusion of different levels of fresh lemon pulp (FLP) was determined as a substitute of sorghum grain in the feeding of hair sheep in parameters of daily weight gain (DWG), daily f...
Population features of Hoplosternum littorale (Hancock, 1828) (Siluriformes, Callichthyidae) at Santo Anastacio Reservoir, Brazil
Objective. The length-weight relationship and condition factor of fish are indicators of physiological well-being and may be related to reproduction and feeding periods. These attributes were evaluated for a population o...
Genetic parameters of type traits and production in Holstein cattle from the Department of Antioquia
Objective. To estimate heritability, phenotypic and genetic correlations among 24 type traits and milk production in Holstein cows from Antioquia. Materials and methods. The data analyzed included 2395 holstein cow recor...
The effect of changing the pre-slaughter handling on bovine cattle DFD meat
Objective. To evaluate the effect that a series of changes in the handling techniques used throughout the pre and post slaughter periods and during the handling of cooled carcass have on the presence of DFD (Dark, firm a...