Biofilm production among coagulase negative staphylococci and its correlation with antimicrobial susceptibility pattern
Journal Title: Medpulse International Journal of Microbiology - Year 2017, Vol 3, Issue 2
Abstract
Background: Coagulase negative staphylococci (CoNS), which are the normal skin flora, have emerged as predominant pathogens in hospital-acquired infections. An important step in the development of catheter or implant-associated infections caused by CONS is the adhesion and attachment of these bacteria to biomaterial surfaces. Biofilms are the microbial communities of the surface-attached cells, which are embedded in a self produced extracellular polymeric matrix. The increased recognition of pathogenic potential of biofilm production in CoNS and emergence of drug resistance among them warrants the need to identify biofilm production and its correlation with antimicrobial susceptibility pattern. Material and Method: Prospective study included a total of 238 non duplicate coagulase negative staphylococci from various clinical specimens during period of Nov 2013 to Oct 2015. All the specimens received were processed further for identification and antimicrobial susceptibility testing by standard microbiological procedures. Biofilm production among CoNS was detected by using two methods. Results: A total of 97 isolates were biofilm producer. Biofilm production by tube method and Congo red agar method was 92 (38.65%) and 48 (20.17%) respectively. Antimicrobial resistance to most of the commonly used antibiotics was more among biofilm producer CoNS as compared to non producers and the difference was statistically significant. Conclusion: Biofilm production in CoNS is of clinical significance as biofilm constitutes reservoir of pathogens and are associated with resistance to antimicrobial agents and chronic infections. So test for detection of biofilm production should be done routinely in laboratory.
Authors and Affiliations
Chincholkar V V, Lomte A A, Nilekar S L, More S R
Keywords
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