BIOFILMS- SURVEILLANCE IN A HEALTHCARE FACILITY AND EFFECT OF A BIOCIDE
Journal Title: Journal of Evidence Based Medicine and Healthcare - Year 2019, Vol 6, Issue 11
Abstract
BACKGROUND Biofilms form in environment, indwelling medical devices and industries. They are responsible for nosocomial infections. They can be detected in-vitro. Various biocides are being evaluated to remove them. We isolated 148 bacteria from 82 swabs collected from the hospital environment of which 37.2% were biofilm producers. MATERIALS AND METHODS Bacterial profile showed CONS (31.1%) and K. pneumoniae (25.7%) to be predominate with 41.3% and 55.3% biofilm producers respectively. Strong biofilm producers were K. pneumoniae (57.1%), CONS (52.6%), Pseudomonas aeruginosa (50%) and Acinetobacter baumannii. (50%). After thorough cleaning with 1% sodium hypochlorite, the number of bacteria isolated was reduced to 54 from 82 samples with 42.6% biofilm producers. RESULTS Data analysis showed that biofilm producing organisms persisted as K. pneumoniae (57.1%), CONS (54.5%), Pseudomonas aeruginosa (40%). Even though 1% sodium hypochlorite showed reduction in bacterial load in the hospital environment, it did not exterminate the biofilm forming organisms. CONCLUSION We observed that with 1% sodium hypochlorite, along with mechanical rubbing, we could not eliminate the biofilm forming organisms. Thus, we need to study spectrum of biocides and their contact time against the biofilms in the hospital environment to effectively control them.
Authors and Affiliations
Ketaki Prakash Ghatole
Keywords
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- EP ID EP615600
- DOI 10.18410/jebmh/2019/192
- Views 83
- Downloads 0
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