[b] An Efficient and Cost Effective Protocol for In Vitro Propagation of Pineapple[/b]
Journal Title: Journal of Ornamental and Horticultural Plants - Year 2013, Vol 3, Issue 4
Abstract
An efficient and cost effective protocol for in vitro propagation of Pineapple (Ananas comosus var. Queen) has been developed. In the proliferation stage, agar based Murashige and Skoog (MS) media was supplemented with 3.0 mg/l benzyleaminopurine (BAP), 0.5 mg/l indole acetic acid (IAA) and 50 mg/l adenine sulphate as RBC design experiment. Two approaches were taken to reduce the chemical cost of micropropagation media. Analytical grade sucrose was successfully replaced by commercial sugar, completely during proliferation stage and up to 66% during rooting stage. Again during the rooting stage, agar based solid media was replaced by liquid media (MSmedia). Bio-degradable Coir and Luffa were used as supporting matrix. As supporting matrix in rooting media, Luffa was found to be more effective. The clonal fidelity of in vitro raised plantlets was confirmed by RAPD technique.
Authors and Affiliations
Ipsita Dutta, Joyita Bhadra, Pritha Ghosh, Babita Saha, Siraj Datta
Keywords
Related Articles
- EP ID EP115605
- DOI -
- Views 162
- Downloads 0
[b]Effects of 1-MCP and Ethylene on Antioxidant Enzymes Activity and Postharvest Physio-Biochemical Characteristics of Cut Carnation Flower cv. ‘Fortune’[/b]
Carnation (Dianthus caryophyllus L.) is one of the most important cut flowers in the world. The majority of the carnation cultivars are sensitive to ethylene which affected the physiological and biochemical posth...
The Effect of Indole Butyric Acid and the Time of Stem Cutting Preparation on Propagation of Damask Rose Ornamental Shrub
In order to investigate the morphological reactions of cutting of damask rose to IBA (indole butyric acid) in different times, an experiment was done in a factorial experiment based on RCD and three times with thre...
[b]The Effect of Different Polyamines on Some Physiological Traits as ACC Oxidase and Superoxide Dismutase Enzymes Activity in [i]Chrysanthemum morifolium[/i] cv. ‘Bright Golden Ann’[/b]
This experiment was conducted to determine the effect of foliar spray with polyamines on postharvest life of chrysanthemum cut flowers in the horticulture laboratory of Islamic Azad University of Rafsanjan in 2...
Effect of Thidiazuron and Salicylic Acid on the Vase Life and Quality of Alstroemeria (Alstroemeria hybrida L .cv. Modena) Cut Flower
In this research, the effects of thidiazuron pulse treatment and salicylic acid were examined to improve vase life and maintain the quality of Alstroemeria ҅Modena҆ cut flowers. The experiment was done in a factori...
[b]Investigation of the Impact of Benzyladenine and Several Natural Compounds on the Vase Life and Some Qualitative Traits of Tuberose Cut Flowers[/b]
Tuberose (Polianthes tuberosa) is one of the most important cut flowers with fragrant buds. To prolong vase life of tuberose, proper preservative solution applying is necessary. For this purpose, two types of che...