[b] Repercussion of isocyanates exposure on different cellular proteins in human pulmonary arterial endothelial cells[/b]
Journal Title: International Journal of Research in Chemistry and Environment (IJRCE) - Year 2011, Vol 1, Issue 1
Abstract
[i]The endothelium is the thin interior surface of blood vessels normally involved in many aspects of vascular biology; including vasoconstriction, vasodilatation, and angiogenesis can be injured by physical or chemical stress, particularly by the drugs and other environmental toxins. Although, isocyanates have drawn significant attention in the recent past as they react with DNA to produce toxicogenomic insults due to high degree of reactivity and low molecular weight having functional group–N=C=O. Moreover, patho-physiological implications resulting from occupational and accidental exposures of these compounds are yet too elusive. On this premise, a strenuous effort was made to assess the cellular response of methyl isocyanate on cultured human pulmonary arterial endothelial cells (HPAE-26). Study was conducted in different time intervals to evaluate cellular response after exposure to isocyanates using N-succinimidyl N-methylcarbamate, a surrogate chemical substitute to methyl isocyanate (MIC). The role of different cell cycle regulatory proteins: cyclin A and E & cdk2; cell cycle inhibitory proteins: p53, p21& GADD45; DNA damage responsive proteins; ATM, ATR & γH2AX and repair proteins: Mre11, Nbs1 & Rad50 were evaluated through immuno-blotting. Our results demonstrate that isocyanates induced toxicity involved in vascular damage of endothelial cells with subsequent perturb expression of different tumor suppressor and repair proteins concurrently higher expression of Cdk2 and cyclins unveiled the progression of oncogenesis in the cells in-vitro.[/i]
Authors and Affiliations
Panwar Hariom , Mishra Pradyumna Kumar
Keywords
Related Articles
- EP ID EP120234
- DOI -
- Views 105
- Downloads 0
New Efficient Green Methodology for the Synthesis of a Series of Potentially Biologically Active Pyrazoles under Microwave Irradiation
In this work a new effective green methodology has been developed for the preparation of a series of potentially biologically active pyrazoles by the cyclocondensation reaction of different symmetrical as well as unsymme...
[b] [/b]
[i] [/i]
Status of Heavy Metal Concentration in Water and Sediment of River Ganga at Selected Sites in the Middle Ganga Plain
The Pollution of aquatic ecosystem by heavy metals has assumed serious proportions due to their toxicity and accumulative behavior. This paper deals with the measurement of five heavy metals i.e. Cu, Cr, Zn, Ni, and...
Mela on Sustainability of Sagar Island Impact of Gangasagar, West Bengal, India
Sagar Island, the largest delta of Sundarbans island complex with an area of nearly 240 sq. km. and with 1.60 lakh population, defines the south-west boundary of Sundarbans. Due to the complex interplay of natural dynami...
Photolysis of Metribuzin in Water under Direct Sunlight - Identification of Photo-transformation Products by LC-MS-MS Electrospray Tandem Mass Spectrometry and Impact on Aquatic Species (Pseudokirchneriella subcapitata)
Photolysis of a systemic herbicide metribuzin was studied in Milli-Q water and in three different aqueous buffer solutions of pH 5.0, 7.0 and pH 9.0. A commercial formulation of the herbicide having the active strength 1...