Characteristics analysis of HBV serological markers of NAT reactive blood donors under different HBsAg status
Journal Title: Chinese Journal of Blood Transfusion - Year 2023, Vol 36, Issue 1
Abstract
Objective To investigate the characteristics of HBV serological markers of NAT reactive blood donors under different HBsAg status. Methods NAT reactive samples, with HBsAg-, HBsAg+ /retest - and HBsAg+ by single reagent were collected from September 2021 to May 2022 in our laboratory. The TMA non-reactive samples were retested by Roche PCR, then HBsAg, anti-HBs, HBeAg, anti-HBe and anti-HBc were detected by ECLI for statistical analysis. Results A total of 66 samples were collected, among which 55 were HBsAg-/NAT+. The positive rate of anti-HBc, anti-HBs+ anti-HBc, anti-HBe+ anti-HBc was 87.3% (48/55), 43.6% (24/55) and 45.5% (25/55), respectively. The positive rate of anti-HBs was 10.9% (6/55) and the overall negative rate was 1.8% (1/55). In 7 HBsAg+ initially/retest -/NAT+ samples, the positive rate of anti-HBc was 100%(7/7), and the positive rate of anti-HBe+ anti-HBc was 71.4%(5/7). In 4 HBsAg+ /NAT+ samples by single reagent, the positive rate of HBsAg+ anti-HBs+ anti-HBe+ anti-HBc was 50% (2/4), and positive rate of anti-HBe+ anti-HBc was100% (4/4). Samples, not reactive to TMA discriminatory and anti-HBc negative, were also non-reactive to individual PCR retest. There were significant differences in the positive rates of anti-HBe+ anti-HBc between HBsAg-/NAT+ samples and HBsAg+ /NAT+ (single reagent) samples (P<0.05). Conclusion Most HBsAg-/NAT+ blood donors were occult hepatitis B virus infection.The anti-HBe+ anti-HBc positive were correlated with HBV infection status. Non-reactivity discriminated by TMA plus anti-HBc negative do not exclude HBV DNA non-reactivity.
Authors and Affiliations
Dequn NI, Tingting WANG, Ting WANG, Mingrui LI, Weiya CUI, Xin XIE, Qing HE, Suping LI
Keywords
Related Articles
- EP ID EP738468
- DOI 10.13303/j.cjbt.issn.1004-549x.2023.01.017
- Views 85
- Downloads 0
Research progress in clinical application of lymphoplasmapheresis in immune-related diseases
Lymphoplasmapheresis(LPE) is a combination of plasma exchange and lymphocyte separation technology. It can not only remove autoimmune antibodies, but also remove the immune active cells producing these antibodies. At the...
IgA deficiency allergic transfusion reaction and its prevention and treatment strategies
IgA deficiency allergic transfusion reaction often occurs in patients with anti-IgA. When blood products containing IgA are transfused into these patients, it may lead to severe allergic transfusion reactions, which can...
Research progress of IgM-enriched human intravenous immunoglobulin products
Comparing with conventional human intravenous immunoglobulin products (hIVIG), IgM-enriched hIVIG have more advantages in the neutralization of pathogens and related toxins, the regulation of complement activity, secreti...
Analysis on the characteristics of rural blood donation population in Dali Bai Autonomous Prefecture
Objective To explore the status and characteristics of voluntary blood donors in rural areas of Dali Bai Autonomous Prefecture (referred as Dali), and to provide basis for scientific and effective voluntary blood donatio...
Factors affecting the efficacy of platelet transfusion and specificity of HLA-Ⅰ antibodies in tumor patients
Objective To analyze the factors affecting the efficacy of platelet transfusion and the specificity of HLA-Ⅰ antibodies in tumor patients. Methods Tumer patients applied for platelet transfusion from November 2019 to Jul...