Characterization and Invitro Cytotoxicity of T-2 Toxin Isolated from Corn
Journal Title: International Journal of Current Research and Review - Year 2018, Vol 10, Issue 1
Abstract
Aim: Main objective of the study is to characterize and evaluate invitro cytotoxicity of T-2 toxin isolated from corn. Methodology: The fungal strain was isolated by standard blotter method; morphological identification was done using scanning electron microscope (SEM). The fungal strain was identified as F. sporotrichioides based on its cultural and morphological characteristics and internal transcribed spacer (ITS/ITS4) primers. The toxin was characterized using thin layer chromatography (TLC), HPLC and Fourier Transform Infrared Spectroscopy (FTIR) Result: 100ml of culture filtrate yielded 10.126µg of toxin. The functional groups of toxin were identified by (FTIR) method. Invitro cytotoxicity of T-2 toxin was evaluated on human keratinocytes, hepatoma and neuron cell lines. HaCaT and HepG2 cells were found to be more sensitive than SHSY5Y to T-2 toxin exposure at 50-100ng and 100-200ng. Discussion and conclusion: T-2 toxin was extracted and characterized from corn samples, by analytical and molecular methods in addition FTIR analysis verifies the functional groups of T-2 toxin. Further invitro cytotoxicity was evaluated using different human cell lines. Comparably skin and liver cell lines found more sensitive to T-2 toxin exposure. Sensitivity of the cell lines to the toxin at ng may be due to the purity of the toxin
Authors and Affiliations
Dr. Farhath Khanum
Keywords
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- EP ID EP533253
- DOI 10.7324/IJCRR.2018.1019
- Views 134
- Downloads 0
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