Characterization of Cucumber Mosaic Virus From Mint in Tehran Province
Journal Title: International journal of Advanced Biological and Biomedical Research - Year 2014, Vol 2, Issue 4
Abstract
Cucumber mosaic virus (CMV) is one of the causal agents of viral diseases that infected mint (Mentha spp.). CMV is a member of the cucumovirus genus in Bromoviridae family. The virus is naturally transmitted by aphids in a non-persistent manner.During growing season 2007-2008, leaf samples from plants of mint fields with symptoms of viral disease were collected in mint fieldas in Tehran province. The polyclonal antibodies were used to the samples by DAS-ELISA . Some of the tested samples, showed positive reaction against cucumber mosaic virus (CMV) specific antibody. Some of the positive samples which are mechanically inoculated on test plants.Some leaf samples from mint plants with symptoms of yellows and mosaic were showed positived reaction against cucumber mosaic virus specific antibody.To confirm the identify of the virus detected using a specific primer pair of CMV which amplify part of coat protein of CMV . A fragment about 700bp was amplified in Immunocapture reverse transcriptase polymerase chain reaction.(IC-RT-PCR).
Authors and Affiliations
Dorna Forghani| Department Plant Pathology, Faculty of Agricultural Science and Engineering, University of Tehran, College of Agriculture and Natural Resources, Karaj, Iran, Gholamhosein Mosahebi Mosahebi| Department Plant Pathology, Faculty of Agricultural Science and Engineering, University of Tehran, College of Agriculture and Natural Resources, Karaj, Iran, Mina Habibi-Koohi| Department Plant Pathology, Faculty of Agricultural Science and Engineering, University of Tehran, College of Agriculture and Natural Resources, Karaj, Iran
Keywords
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