CHARACTERIZATION OF HIGH ACTIVITY DRUG-STIMULATED ATPase OF MULTIDRUG RESISTANT LEISHMANIA DONOVANI AMASTIGOTE CELLS
Journal Title: International Journal of Research and Development in Pharmacy & Life Sciences (IJRDPL) - Year 2015, Vol 4, Issue 6
Abstract
Background: The emergence of drug resistance and the limited knowledge of the mechanisms by which the parasites acquire resistance are the major obstracle for the control of leishmaniasis. Methods: The present investigation is aimed to study multidrug resistance related ATPase activity in in vitro generated sodium stibogluconate (SSG) and paramomycin (PMM) resistant Leishmania donovani amastigote permeablized by digitonin Results: The results of the multidrug resistance related ATPase activity in SSG and PMM resistant L.donovani amastigotes revealed that ATPase activities were strongly stimulated by resistance causing drugs, and these ATPase stimulation activities had broad specificities. Conclusion: Generation of drug resistance in L. donovani amastigotes leads to concurrent development of drug-stimulated ATPase activity.
Authors and Affiliations
Chowdhury S| Division of Medicinal Biochemistry, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India, Roy A| Division of Medicinal Biochemistry, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India, Kar N| Division of Medicinal Biochemistry, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India, Chakraborty S| Division of Medicinal Biochemistry, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India, Bera T| Division of Medicinal Biochemistry, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India
Keywords
Related Articles
- EP ID EP16032
- DOI -
- Views 406
- Downloads 17
Development and in-vitro evaluation of Furosemide Solid Dispersion using different Water Soluble Carriers
Objective: The objective of the present investigation was to improve solubility and dissolution characteristics of Furosemide, which might offer improved bioavailability. The bioavailability of the drug when taken oral...
Study to assess high demand and high commercial value Medicinal Plants of Jammu and Kashmir India - with special focus on routes of procurement and identification
Despite ancient nature of the tradition, medicinal plants still form the basis of traditional or indigenous health systems and are reported by the World Health Organization (WHO) to still be used by the majority of the...
Comparative utility of C reactive protein and Blood culture for diagnosis of neonatal septicaemia
Introduction: Neonatal septicaemia constitutes a significant cause of morbidity and mortality of neonates in India. The diagnosis of neonatal septicemia based on clinical manifestations is nonspecific which leads to in...
Herbal excipients in Novel Drug Delivery Systems
Due to advances in drug delivery technology, currently, excipients are included in novel dosage forms to fulfil specific functions and in some cases, they directly or indirectly influence the extent and/or rate of drug...
EVALUATION OF ANTI-INFLAMMATORY POTENTIAL OF ETHANOLIC EXTRACT OF THE LEAVES OF RHIZOPHORA MUCRONATA, A SUNDERBAN MANGROVE
Objective: Biochemically unique mangrove plants are recognized worldwide for valuable medicinal actions. Experimental investigations of these pharmacological activities are needed for elucidation of cost effective new t...