Characterization of Pseudocercospora fijiensis Morelet isolates for use in improvement programs for Musa sp.
Journal Title: Biotecnología Vegetal - Year 2004, Vol 4, Issue 2
Abstract
ABSTRACT The present investigation was carried out with the objectives of isolating and identifying Pseudocercospora fijiensis isolates of different regions of Villa Clara and Ciego de Ávila provinces, to characterize them culturally, morphologically and physiologically as well as to evaluate their pathogenicity and virulence on Musa cultivars. The growth in solid and liquid cultures media was evaluated, the structures of asexual reproduction were characterized and the subculture number effect on the conidia production was determined, and the Minimum Inhibitory Concentration (MIC) of Higromicin B and Carbendazim was determined in front of these. P. fijiensis isolates were obtained and their cultural and morphological characteristic coincided with those referred in the scientific literature for this specie. It was also proven, that the in vitro conidia production diminished with the increment of the subcultures number and that it was independent of the isolates. It was possible to determine the MIC of Higromicin B and Carbendazim with the agar dilution method using mycelia suspensions as inoculums. It was demonstrated their pathogenicity on Musa genotypes artificially inoculated with mycelia suspensions and there were differences in respect their virulence. The obtained results evidenced the variability of the M. fijiensis by their sexual reproduction and the necessity of their study to find effective strategies for the plants improvement.
Authors and Affiliations
Mileidy Cruz Martín, Yelenys Alvarado Capó, Mayra Acosta Suárez, Michel Leiva, Berkis Roque
Keywords
Related Articles
- EP ID EP684324
- DOI -
- Views 145
- Downloads 0
Effect of explant density and volume of culture medium on the in vitro propagation of the hybrid cultivar 'FHIA-21' (AAAB) in Temporary Immersion Systems
The need of producing high quality planting material has required the searching of new alternatives to increase the efficiency of in vitro propagation methods and their automation, such as Temporary Immersion Systems. T...
The effects of plant growth promoting Bacillus pumilus CCIBP- C5 on ‘Grande naine’ (Musa AAA) plants in acclimatization stage
The search of effective and sustainable technological alternatives to replace chemical fertilization it is a challenge for scientists. Positive actions of microorganisms on plants growth are known. Considering these cr...
In vitro establishment and multiplication of Aloe vera L. shoots
The high demand of Aloe vera L. in the pharmaceutical and cosmetic industries has caused that the traditional crop is insufficient to satisfy the current market. In Cuba, this plant is used as a constituent of many pha...
Effect of 60Co gamma radiation on plant regeneration of Phaseolus vulgaris L. cultivar 'ICA Pijao' via indirect organogenesis
In Phaseolus vulgaris L. breeding programs has been used mutation induction, however, no radiosensitivity studies have been performed in vitro in order to define the optimal dose of radiation to be applied. The aim of...
In vitro establishment of Handroanthus heptaphyllus nodal segments
Micropropagation could be used as a viable alternative in the mass production of Handroanthus heptaphyllus (Vell.) Mattos (white lapacho). The objective of the present work was to establish in vitro of nodal segments of...