Characterization of Salmonella isolates from municipal sewage, patients, foods, and animals in Greece using antimicrobial susceptibility testing and pulsed field gel electrophoresis
Journal Title: International Journal of One Health - Year 2016, Vol 2, Issue
Abstract
Aim: We aimed to compare Salmonella isolates from different sources using molecular and phenotypic methods, targeting better possibility of understanding the epidemiology of this organism in the Greek context with emphasis in municipal wastewater. Materials and Methods: In this study, we used pulsed field gel electrophoresis (PFGE) in combination with antimicrobial susceptibility testing to analyze a total of 88 Salmonella Enterica isolates from municipal sewage (n=25), humans (n=36), animals (n=24), and foods (n=3) in Greece. Results: The higher resistance rates were found to the following antimicrobials: streptomycin (59.1%), tetracycline (47.7%), nalidixic acid (46.6%), ampicillin (37.5%), and oxolinic acid (35.2%). Resistance to ciprofloxacin was not observed; 22 isolates (25%) were sensitive to all 9 antimicrobials, 36%, 25% and 12% of human, animal and wastewater origin, respectively, showing a significant difference. Salmonella ser. Hadar was the serovar with the highest resistance rates followed by Salmonella ser. Anatum and Salmonella ser. Typhimurium; Salmonella ser. Infantis strains were almost pansusceptible. Cluster analysis did not reveal close genetic relationship between human animal food and wastewater strains belonging to the same serovars. In most of the cases, distinct clusters were observed between human and non-human isolates indicating diversity and no epidemiological connection. Conclusion: This study indicates that municipal wastewater would be of interest to further monitor the community's prevalence of subclinical or non-reported S. Enterica infections.
Risk factors associated with cystic echinococcosis in humans in selected pastoral and agro-pastoral areas of Uganda
Aim: It was to determine the risk factors responsible of occurrence of cystic echinococcosis (CE) of humans in the pastoral and agro-pastoral (PAP) in Uganda. Materials and Methods: A cross-sectional study was conducted...
Brucellosis: Community, medical and veterinary workers' knowledge, attitudes, and practices in Northern Uganda
Aim: This study aimed at determining the knowledge, attitudes, and practices of the community, medical and veterinary workers regarding brucellosis. Materials and Methods: A cross-sectional study was conducted at select...
Emergence of extended spectrum beta-lactamases-producing strains belonging to cefotaxime-M-1 class from intensive care units patients and environmental surfaces in Pakistan
Aim: The emergence of multidrug-resistant (MDR) bacteria is the most dangerous threat for the treatment of infectious diseases. The aim of this study was to detect and characterize extended spectrum beta-lactamases (ESBL...
Prevalence and risk factors associated with brucellosis among high-risk individuals in Lafia, Nasarawa state, Nigeria
Background and Aim: Brucellosis caused by Brucella species is a zoonotic disease prevalent in Nigeria. Data on this disease in humans and its risk factors in Lafia, Nasarawa State, are lacking. This study was, therefore,...
Depiction of global trends in publications on mobile health
Background: Variety of mobile health initiatives in different levels have been undertaken across many countries. Trends of these initiatives can be reflected in the research published in m-health domain. Aim: This paper...