Characterization of Vaginal Lactobacillus Strains and Their Potential Antagonistic Effects on Candida albicans
Journal Title: Microbiology Research Journal International - Year 2015, Vol 6, Issue 4
Abstract
Aims: To characterize Lactobacillus strains for potential beneficial effects, especially for inhibition of Candida albicans growth. Materials and Methods: Twelve vaginal Lactobacillus strains belonging to five species from our laboratory collection were studied. The acid production and hydrogen peroxide (H2O2) production were determined. The biofilm formation ability was measured by a semi-quantitative microtiter plate assay. Besides the detection of autoaggregation and coaggregation capacities with C. albicans using a spectrophotometric assay, aggregating clusters were observed by light microscopy. The spot inhibition, agar overlay and Oxford Cup methods were used to test antagonistic activities of Lactobacillus strains against C. albicans. Results: Seven strains of L. fermentum (A011, A024, A025, A035, A041, A044 and A081) and L. salivarius A023 were revealed to decrease the medium pH below 4.5 just within six hours due to the rapid growth. L. jensenii A083 and L. gasseri A054 were the best biofilm formers in polystyrene plates, whereas the other ten strains could form biofilm with varying affinities. In addition to significant H2O2 production found in L. jensenii A083, L. crispatus A014 and A055, these strains showed strong autoaggregation and coaggregation abilities with C. albicans (ATCC 90029 and Z03). L. salivarius A023, L. fermentum A025 and L. crispatus A014 displayed strong activities against C. albicans in spot inhibition and agar overlay assays. Conclusion: The potential beneficial properties of Lactobacillus spp. were strain specific. Among the tested strains, L. salivarius A023, L. fermentum A025 and L. crispatus A014 might be better candidates for further investigation on the inhibition of C. albicans growth.
Authors and Affiliations
Shuai Wang, Qiangyi Wang, Bingbing Xiao, Rui Zhang, Ben Wang, Qinping Liao, Hui Zhuang, Tong Li
Keywords
Related Articles
- EP ID EP355000
- DOI 10.9734/BMRJ/2015/15116
- Views 107
- Downloads 0
Evaluating Efficacy of Biosurfactants from Bacterial Isolates in Conferring Protection against Rhizoctonia and Sclerotium Infection in Wheat and Peanut Plants
Aims: To isolate biosurfactant producers from natural habitat and to test the antimicrobial activity of the extracted biosurfactant against fungal plant pathogens. Study Design: The present study was designed to achieve...
Heavy Metal Tolerance Profile among Bacteria from Auto-mechanic Workshop and Pristine Soil
The study was aimed at investigating the heavy metal tolerance profile among bacteria from auto-mechanic workshop and pristine soil samples. Auto-mechanic workshop and pristine soil environments were randomly sampled wit...
Multiple Antibiotic-resistant Escherichia coli in Ready-to-eat Foods from Food Outlets in Ekiti State University and Its Environ
The potential health risk associated with consumption of ready-to-eat (RTE) food sold in food outlets in and around Ekiti State University was investigated using standard techniques. The microbiological analysis of 96 fo...
Antidermatophytic Activities of Musa sapientum Methanol Leaf Extract in-vitro
Aim: The in vitro antidermatophytic activity of methanol leaf extract of Musa sapientum (MSL) against Microsporum canis, Trichophyton tonsurans and Trichophyton rubrum was investigated. Methodology: The methanol leaf e...
Antimicrobial Resistance of Pathogenic Bacteria Isolated from Mastitis Cows in Khartoum State, Sudan
Aims: The problem of antimicrobial resistance (AMR) is now recognized as a major threat to the health and development in all countries. This study was conducted to determine pathogenic bacteria asso...