CHEMICAL COMPOSITION, ANTIMICROBIAL, AND ANTIOXIDANT ACTIVITIES OF TWO MOROCCAN THYMUS ESSENTIAL OILS
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2019, Vol 12, Issue 1
Abstract
Objective: The aim of the present study is to investigate chemical constituents of Thymus riatarum and Thymus blecherianus essential oils (EOs) as well as to evaluate, for the first time, their antioxidant effect and antibacterial activity against six bacterial strains responsible for nosocomial infections.Methods: The chemical composition of EOs was analyzed using gas chromatography (GC) and GC-mass spectrometry, the antibacterial capacity of the two thymus species was evaluated against six bacteria species: Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, Staphylococcus aureus, Acinetobacter baumannii, and Citrobacter sp. using disk diffusion method and microdilution assay. Finally, the antioxidant activity was measured by four different test systems of assay, namely free radical scavenging activities, ferric reducing/antioxidant power assays, total phenolic, and flavonoid contents.Results: A total of 15 compounds representing 99.6% of T. riatarum oil were identified with thymol (28.8%), borneol (20.0%), and α-phellandrene (13.0%) as the main constituents. Eleven components of T. blecherianus were identified representing 98.2% of the total oil composition; the most abundant constituents were as follows: Carvacrol (45.9%), bornyl acetate (20.1%), and borneol (15.7%). Strong antibacterial activity of the two EOs was identified against all bacterial strains tested. Concerning the antioxidant results, T. riatarum EO exhibited higher antioxidant activity than T. blecherianus in the three assays with an IC50 value equal to 5.75±0.06 mg/ml, which was probably due to its high content of polyphenols (28.95±0.13 mg GAE/g DW). Total flavonoid content was found equal for the two EOs.Conclusion: EO of T. riatarum and T. blecherianus from Morocco can be exploited as a natural antibacterial and antioxidant new potential sources.
Authors and Affiliations
Imane Aouam, Yassine El Atki, Amal Taroq, Fatima El Kamari, Abdelfattah Abdellaoui
Keywords
Related Articles
- EP ID EP607181
- DOI 10.22159/ajpcr.2019.v12i1.30377
- Views 120
- Downloads 0
UTILITY OF ANTIGEN DETECTION TEST AND POLYMERASE CHAIN REACTION IN THE DIFFERENTIATION OF TUBERCULOUS AND NON-TUBERCULOUS MYCOBACTERIA
Objectives: Cultivation and identification of mycobacteria to species level remains difficult and time-consuming. Hence, easy and rapid diagnostic methods are necessary for the differentiation of Mycobacterium tuberculos...
TOPICAL APPLICATION OF EUGENIA CARYOPHYLLUS OIL AGAINST RINGWORM INFECTION OF HUMAN BEINGS
Objective: At least one million people are suffering from dermatophytosis in India. These mycoses although normally not lethal are unpleasant, frequent disease recurrence, and resistant dermatophytic strains cause consid...
PHYTOCHEMICAL SCREENING AND ANALGESIC, ANTI-BACTERIAL AND CYTOTOXIC ACTIVITY EVALUATION OF ETHANOL EXTRACT OF PITHCELLOBIUM DULCE ( ROXB.) BENTH LEAF
 Introduction: Pithcellobium dulce (Roxb.) benth, tree 5-18 m high, with pendulous branches, short, sharp stipular spines. It belongs to Fabaceaefamily. This plant’s accession number is 31,388 as per Bangladesh nation...
ANTIBIOTIC UTILIZATION PATTERN AT THE SURGERY DEPARTMENT OF A TERTIARY CARE HOSPITAL
Objective: The present study aims to determine the pattern of antibiotic utilization at the surgery department of a tertiary care hospital.Method: A prospective observational study was conducted over a period of 6 months...
THE CYTOPROTECTIVE ACTIVITY OF AQUEOUS GREEN TEA EXTRACT AGAINST METRONIDAZOLE AND TINIDAZOLE GENOTOXIC EFFECT
Objective: The study was designed to evaluate the genoprotective effect of green tea extract against genotoxicity induced by metronidazole and tinidazole.Methods: A total of 36 mice were used, for each experiment. The an...