Chemical profile and antimicrobial activity of Secondatia floribunda A. DC (Apocynaceae)
Journal Title: Asian Pacific Journal of Tropical Biomedicine - Year 2017, Vol 7, Issue 8
Abstract
Objective: To establish the chemical profile, and to evaluate the antibacterial and modulatory activity of the ethanolic extracts of the stalk's inner bark and heartwood of Secondatia floribunda. Methods: Quantification of total phenols and flavonoids was determined by the Folin- Ciocalteu method and aluminum chloride, respectively. Phenolic compounds were identified and quantified by HPLC-DAD (High Performance Liquid Chromatography- Diodearray Detector) and the Infrared Spectroscopy was performed using the measure by Attenuated Total Reflectance with Fourier Transform (ATR-FTIR). Antibacterial assays for determination of the Minimum Inhibitory Concentration (MIC) and modification of aminoglycosides were performed by microdilution. Results: Infrared spectra showed similar characteristics, having among its main absorption bands hydroxyl group (OH). The antibacterial activity showed clinically significant results for the strains of Staphylococcus aureus and Escherichia coli. In modulation assay, synergic and antagonistic effect for both extracts was observed. Heartwood extract in combination with antibiotics showed a significant MIC reduction at 19.8% (P < 0.000 1)-79.3% (P < 0.01). Conclusions: This study is the first report of chemical and biological information of Secondatia floribunda suggesting that it is clinically relevant source of a new antibacterial therapy, especially due to the presence of significant levels of phenolic compounds.
Authors and Affiliations
Daiany Alves Ribeiro, Sarah Soares Damasceno, Aline Augusti Boligon, Irwin Rose Alencar de Menezes, Marta Maria de Almeida Souza, José Galberto Martins da Costa
Keywords
Related Articles
- EP ID EP269678
- DOI 10.1016/j.apjtb.2017.07.009
- Views 64
- Downloads 0
Therapeutic effect of soluble worm protein acting as immune regulatory on colitis
Objective: To investigate the anti-inflammatory effect of the protein derived from the soluble factor of Heligmosomoides polygyrus (H. polygyrus) excretory-secretory in a colitis model. Methods: Colitis was induced by pr...
Comparative assessment of intestinal helminths prevalence in Water, Sanitation and Hygiene (WASH) intervention and non-intervention communities in Abeokuta, Nigeria
Objective: This study compared the prevalence of intestinal helminths in Water, Sanitation and Hygiene (WASH) intervention and non-intervention communities in Abeokuta, Nigeria. Methods: Stool samples were collected from...
Contribution of public places in proliferation of dengue vectors in Penang Island, Malaysia
Objective: To determine abundance, distribution and diversity of potential breeding container habitats of the dengue vectors in public places including schools, restaurants, mosques and parks in southwest areas of Penang...
Flavonoid chemical composition and antidiabetic potential of Brachychiton acerifolius leaves extract
Objective: To evaluate Brachychiton acerifolius leaf extracts as antidiabetic potential agent and to identify the main active constituents using bioactivity guided fractionation. Methods: In vitro antioxidant activity wa...
Red propolis: Chemical composition and pharmacological activity
Propolis has been used worldwide for years in folk medicine and currently marketed by the pharmaceutical industry. In Brazil, propolis was classified into 13 groups based on their organoleptics and physicochemical charac...