Clinical Significance of FLT-3 Internal Tandem Duplication and D835 Mutations in Acute Myeloid Leukemia Patients from West Indian Population
Journal Title: GCSMC Journal of Medical Sciences - Year 2017, Vol 6, Issue 1
Abstract
Introduction: Methods: Results Conclusion: The FMS-like tyrosine kinase-3 (FLT3), a member of the Platelet-derived growth factor (PDGF-R) subfamily of receptor tyrosine kinases, expressed on early hematopoietic progenitor cells play an essential role in survival and differentiation of stem cell. Majority of acute myeloid leukemia (AML) patients have mutation in this gene. Two types of frequent mutations are present in this gene. Both the types FLT3-ITD and D835 mutations play an important role in prognosis of AML patients. Total 33 patients were enrolled in the study. Blood samples were collected from the subjects, from which the DNA isolation was carried out.For FLT3-ITD mutation, PCR was performed and for D835 mutation PCR-RFLP was performed. DNA segments were amplified using Polymerase Chain Reaction (PCR). Results: FLT-3 ITD mutation was detected in 12% of patients and D835 mutation was detected in 3% of patients. The study revealed significant correlation between ITD and Tdt, while D835 negatively correlated with CD33, HLADR and Tdt. However, there was no substantial correlation of D835 with LDH value. Results also revealed that FLT-3 ITD significantly correlated with LDH values in AML patients. The mean value of LDH was 753.45 IU/L in ITD positive patients as compared to ITD negative patients with 338 IU/L mean LDH value, suggesting higher LDH values in ITD positive. These Genotypic analysis of FLT-3 mutation results from West Indian population provide important tools for understanding of AML pathogenesis and determination of appropriate therapeutic intervention. Further large number of patient data can also corroborate these significant results.
Authors and Affiliations
Sabrina Pathan, Kinjal R Patel, Asha Anand, Prabhudas Patel, Jayendra B Patel
Keywords
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