Clinical Significance of Glutathione S-transferase Enzymes in Acute Leukaemia Patients
Journal Title: GUJARAT CANCER SOCIETY RESEARCH JOURNAL - Year 2015, Vol 17, Issue 1
Abstract
Leukemic cells produce higher amounts of reactive oxygen species (ROS) than non-leukemic cells as they are under a repeated state of oxidative blockade. Antioxidant defence system such as glutathione-S-transferase (GST) and glutathione reductase (GR) counteracting ROS induced oxidative stress. Therefore, the study was aimed to evaluate the role of antioxidant defence system in leukemogenesis. Twenty five untreated acute leukaemia patients and twenty five healthy individuals were enrolled. Plasma GST, GR and total thiol levels were measured using highly sensitive spectrophotometric methods. The prevalence of GSTM1 polymorphism was examined using polymerase chain reaction (PCR). Statistical analysis was done using SPSS version 13. Acute leukaemia patients showed significantly higher GST and GR activities (p=0.052 and p=0.001 respectively) whereas thiol levels were significantly lower (p=0.001) as compared to controls. Mean GR activity was found to be significantly higher (p<0.05) in acute myeloid leukaemia patients than acute lymphoid leukaemia patients. Correlation analysis revealed positive correlation between GST and GR, and negative correlation between GR and thiol levels. Receiver’s Operating Characteristic (ROC) curve analysis revealed that GR activity and total thiol levels were highly efficient to discriminate between acute leukaemia patients and controls. 60% of acute leukaemia patients showed GSTM1 null genotype, whereas only 25% of controls demonstrated GSTM1 null genotype. In Conclusion, increased GST and GR activity, and decreased thiol levels were observed in acute leukemia. Moreover, GSTM1 null genotype was also associated with increased risk of acute leukemia patients. These results suggested significant role of GST, GR and thiol in acute leukemia patients.
Authors and Affiliations
Jayendrakumar Patel, Franky Shah, Kinjal R Patel, Bhairavi Vajaria, Shruti Patel, Kinjal D Patel, Prabhudas Patel
Keywords
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