CLINICO-MYCOLOGICAL STUDY OF DERMATOPHYTIC SKIN INFECTIONS IN A TERTIARY CARE CENTRE
Journal Title: Journal of Evolution of Medical and Dental Sciences - Year 2018, Vol 7, Issue 8
Abstract
BACKGROUND Skin infections are the most common infections occurring in developing countries, of which dermatophytosis are of particular concern in tropics. The prevalence of dermatophytic infections varies with different geographic areas and climatic conditions. Hence, identification of aetiological agents is important for diagnosis and treatment as well as for studying epidemiological characteristics in a region. Objectives1. To isolate and identify the dermatophytes infecting skin. 2. To compare two different culture media, namely Sabouraud’s Dextrose Agar (SDA), Hi-Media Laboratories, Mumbai) with Actidione and Dermatophyte Test Medium (DTM, Hi-Media Laboratories). MATERIALS AND METHODS This is a cross-sectional study conducted in patients attending the Outpatient Department of Dermatology and Venereology, Government Medical College, Kottayam, Kerala, India, with clinical features of dermatophytic skin infections from January 2009 - December 2009. Skin scrapings were subjected to direct microscopy by 10% potassium hydroxide (KOH) and cultured on SDA with Actidione (Hi-Media Laboratories) and DTM (Hi-Media Laboratories). RESULTS The total number of samples in this study was 115, of which 76 (66.1%) samples were positive by direct microscopy and 50 (43.5%) were positive by culture. The most common clinical type was tinea corporis 46 (40%). Out of the 50 isolates, Trichophyton rubrum 24 (48%) was the most common species followed by Trichophyton mentagrophytes 20 (40%), Epidermophyton floccosum 4 (8%), Trichophyton violaceum 1 (2%) and Microsporum gypseum 1 (2%). Nearly, 85.4% of the dermatophytes were isolated on DTM within 5 - 10 days of incubation, whereas 45.7% were isolated on SDA with Actidione within 5 - 10 days of incubation. CONCLUSION DTM can be used as a rapid screening medium for the isolation and identification of dermatophytes compared to SDA with Actidione. But DTM is inferior to SDA with Actidione for species level identification of dermatophytes.
Authors and Affiliations
Shanimole Puthenpurayil Ebrahimkutty, Shoba Kurian Pulikkottil, Dani Kochuplapparambil Thampi
Keywords
Related Articles
- EP ID EP410100
- DOI 10.14260/jemds/2018/230
- Views 95
- Downloads 0
AN ATTEMPT TO LOCATE THE TRANSVERSE AXIS OF TEMPOROMANDIBULAR JOINT BY ADAPTING A NEW METHOD
The present study is aimed to ascertain the position of transverse axis of temporomandibular joint in living subjects by devising a new method, which remains hypothetical till date with a hope that it can help the biomec...
COEXISTENCE OF CUTANEOUS MUCORMYCOSIS AND SCROFULODERMA IN AN IMMUNODEFICIENT CHILD
Severe Combined Immunodeficiency (SCID) is a genetic disorder characterised by the disturbed development of functional T cells and B cells caused by numerous genetic mutations. (1) These patients are extremely v...
COMPARISON OF SUPRAGLOTTIC AIRWAY DEVICE I-GEL WITH CLASSIC LARYNGEAL MASK AIRWAY AND PROSEAL LARYNGEAL MASK AIRWAY FOR SHORT SURGICAL PROCEDURES
BACKGROUND Supraglottic airways are airway devices for providing ventilation, oxygenation and anaesthetic gases to the patients. The SGA has revolutionised airway management because of its ease of insertion, hands-free o...
BONE MARROW EXAMINATION- A TWO-YEAR STUDY AT A TERTIARY CARE CENTRE
BACKGROUND The incidence of haematological diseases is quite alarming in Indian population. The commonest presenting symptom in most of these conditions is anaemia. Apart from peripheral blood examination bone marrow ana...
A NEGLECTED CASE OF PRIMARY INFERTILITY – A CASE REPORT OF DISSEMINATED TUBERCULOSIS
Tuberculosis (TB) is a major health problem in developing countries including India and genital tuberculosis (GTB) is responsible for infertility in a significant proportion of these women. Inspite of recent ad...