Cloning, Expression and Functional Characterization of the D-Amino Acid Oxidase from Rhodosporidium Diobovatum
Journal Title: International Journal of Engineering and Science Invention - Year 2018, Vol 7, Issue 5
Abstract
D-Amino Acid Oxidases(DAAO) Catalyze The Enantioselective Oxidation Of A Broad Variety Of D-Amino Acids To Their Corresponding Α-Amino Acids, Which Spontaneously Hydrolyze To Α-Keto Acids And Ammonium. The Enzyme Of Some Species Has Been Reported. But, In This Study, The Complete Nucleotide Sequence Of The DAAO In The Basidiomycetes Rhodosporidium Diobovatum Has Been Determined. The DAAO Gene Of R. Diobovatum Was Isolated Using Methods Of RACE And RT-PCR. The Results Showed That The Encoded Polypeptide Holds A Sequence Of 357 Amino Acid Residues With Homology To Those Of DAAO From Other Yeasts. The Deduced Protein Molecular Weight Is 38.33 Kda, Theoretical Isoelectric Point Is 7.60. The Expression Vectors Pet-DAAO Was Constructed And Expressed In Escherichia Coli. D-Amino Acid Oxidase Activity On Cephalosporin C Showed A Maximum Around 24℃ And The Optimum Reaction Ph Range From 7.5-8.5.
Authors and Affiliations
Wen Xu, Min Kong, Hongpeng Wang, Hui Tang, Liping Zhang
Keywords
Related Articles
- EP ID EP396966
- DOI -
- Views 82
- Downloads 0
Different Deterioration Rates Two Warehouse Production Inventory Model with Time and Price Dependent Demand under Inflation and Permissible Delay in Payment
A two warehouse production inventory model with different deterioration rates under inflation and permissible delay in payments is developed. Demand is considered as function of price and time. Holding cost is considered...
Cocoon Storage: A Better Alternative to the Use of Inorganic Insecticides/Pesticides in Middle Level and Large Scale Storage, in the Tropics
This paper reviews the economics and efficacy of hermetic cocoon storage as a preferred alternative to use insecticides and pesticides for middle level and large scale storage of cereal grains, paddy crops, oil seeds and...
Ground Water Quality Analysis and Mapping of District Nuh and Its Surrounding Ecosystem: A Case Study of Nuh, Haryana
Water, the elixir of life, is a natural resource of utmost importance. Nuh district being a paramount water resource for the Mewat region has been subjected to uncontrolled wastewater disposal. Its 30-km stretch passing...
Present Status of Insecticides Use for The Cultivation of Brinjal in Kushtia Region, Bangladesh
This study has been carried out to assess the present status of insecticides use for the cultivation of brinjal in Kushtia region. A total number of 240 brinjal growers were randomly selected and interviewed by structure...
Gasification of Lafia-Obi Coal using ANSYS Fluent as CAD Tool and Effect of Temperature and Pressure on Producer Gas Composition
Gasification, the thermo-chemical conversion of fuels to combustible gases has assumed prominence because of its many advantages which include greenhouse gas sequestration, controllability among others. In this paper, La...