Cloning of synthetic gene including antigens against Urinary Tract Infections in pET28a+ vector
Journal Title: Journal of Pharmaceutical & Health Sciences - Year 2017, Vol 5, Issue 3
Abstract
There are many different bacterial infections in the world that patients are suffering from and research teams are trying to find suitable ways to prevent and treat them. Urinary Tract Infections (UTIs) are most important infections in the world , and they are more common among women because vaginal cavity is near to urethral opening. The aim of this study is cloning of synthetic gene include antigens against UTIs in pET28a+ vector. Antibiotic resistant has been increasing because of antibiotic overuse recently, so It shows the necessity of developing a vaccine against these infections. There for, it will be imperative to develop a vaccine instead of antibiotics. This infection causes by many organisms, most important of which are Uropathogenic Escherichia coli (UPEC), Proteus mirabilis and Klebsiella pneumoniae Uropathogenic Escherichia .coli is the most important microorganism that causes these infections more than other bacteria, so in developing a vaccine it is the most important one, that have to be considered. The synthetic Gene which was designed against these three bacteria including antigens which are important and common to cause these infections. This gene has involved 1293bp. It was ordered to Gene Ray Biotechnology. Primers were designed by Gene Runner. Gene and pET28a+ vector was checked by SnappGene. Synthetic gene was multiplied by PCR and cloned in pET28a+ vector. Construct was transformed into E. coli TOP10.The clone was confirmed by PCR, Digestion. This data indicates that this gene can be expressed and it might be a vaccine candidate to protect people from these infections in the future.
Authors and Affiliations
Zohreh Haghri, Jamil Kheirvari Khezerloo, Masoum Amraee, Afsaneh Yavari
Keywords
Related Articles
- EP ID EP242989
- DOI -
- Views 151
- Downloads 0
Comparison the effect of carboxymethyle cellulose films containing Thymus vulgaris and Zataria multiflura on shelf life of chicken meat
Poultry meat is a kind of perishable food. The growth of pathogenic microorganisms may occur in chicken meat during storage in the refrigerator. Microbial growth causes a serious hazard to the safety of the food consumer...
Pharmacognostic Study of Argyreia pilosa Wight & Arn. Root
Background: Ethnomedicinally, the plant Argyreia pilosa Wight & Arn. (Convolvulaceae) has long been utilized in various disorders in the conventional system; most significantly it is utilized against sexually transmitted...
Cloning of synthetic gene including antigens against Urinary Tract Infections in pET28a+ vector
There are many different bacterial infections in the world that patients are suffering from and research teams are trying to find suitable ways to prevent and treat them. Urinary Tract Infections (UTIs) are most importan...
Cloning and determination of biochemical properties of protective and broadly conserved vaccine antigens from the genome of extraintestinal pathogenic Escherichia coli into pET28a vector
Urinary tract infections are one of the most common infectious diseases that lead to significant health problems in the world. Urinary tract infections are referred to any infection in any part of the renal system. Uropa...
Preparation of ZnO and ZnS nanoparticles and in-vitro study of their antimicrobial effect
Zinc sulfide (ZnS) & zinc oxide (ZnO) nanoparticles was evaluated for their antimicrobial activity against four pathogenic strains. ZnS&ZnO nanoparticles were synthesized by simple aqueous chemical reaction in an aqueous...