COMPARATIVE ANALYSIS OF ANTI-INFLAMMATORY ACTIVITY OF AQUEOUS AND METHANOLIC EXTRACTS OF C. CASSIA AND C. ZEYLANICUM IN RAW264.7, SW1353 AND PRIMARY CHONDROCYTES
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 11
Abstract
Objectives: The objective of this research was to compare the anti-inflammatory activity of aqueous and methanolic extracts of C. cassia (CC) and C. zeylanicum (CZ) in mouse macrophage (RAW264.7) and human chondrosarcoma (SW1353) cell lines as well as in human primary chondrocytes, to correlate their efficacy in management of osteoarthritis (OA) related pathophysiology.Methods: RAW264.7, SW1353 and human primary chondrocytes were pre-treated with aqueous extracts of C. cassia (CCW) and C. zeylanicum (CZW) and methanolic extracts of C. cassia (CCM) and C. zeylanicum (CZM) at various concentrations (0.1-100 µg/ml) for 1 h, followed by stimulation with LPS and IL-1β, respectively. The effect of CCM, CCW, CZM and CZW on the production of nitric oxide (NO) was evaluated by Griess reaction. Evaluation of prostaglandin E2 (PGE2) and leukotriene (LTB4) proteins was performed by EIA-Monoclonal based kits. The effect of these extracts on matrix metalloproteinase (MMPs-2, 9 and 13) levels was analyzed by SensoLyte® fluorimetric MMP assay kit.Results: The methanolic extracts (CCM, CZM) of both the varieties of cinnamon were found to be more effective than the aqueous extracts in terms of PGE2, LTB4 and MMP inhibition.We found that in RAW 264.7, CCM and CZM decreased NO and PGE2 production by45.40%±8.6; 65.63%±5.7 and 79.88%±1.2; 95.91%±0.3, respectively. Similarly, in SW1353 and chondrocytes, CCM decreased PGE2 production by 68.8%±6.4;36.1%±9.5, respectively whereas CZM reduced PGE2 production by 70.2%±2.3; 52.3%±5.4, respectively. Moreover, in SW1353 and chondrocytes CCM decreased LTB4 production by 85.47%±3.03; 99.6%±0.2, respectively whereas CZM reduced LTB4 production by 67.5%±5.6; 75.6%±1.2, respectively. In chondrocytes both CCM and CZM significantly reduced the levels of MMP-2(55.7%±5.2; 73.1%±7.1), MMP-9 (57.5%±4.7; 74.5%±5.2) and MMP-13 (90.1%±2.6; 71.2%±12.5), respectively. However, on comparing the two species of cinnamon, C. zeylanicumwas found to be more effective than C. cassia andthus could be considered for its potential therapeutic application in the management of inflammatory conditions associated with OA.Conclusion: The present study would help in choosing better of the two species of cinnamon for their possible therapeutic application in the management of inflammatory condition associated with OA.Â
Authors and Affiliations
Prerna Raina, Cv. Chandrasekaran, Amit Aggarwal, Narendra Wagh, Ruchika Kaul Ghanekar
Keywords
Related Articles
- EP ID EP578196
- DOI -
- Views 52
- Downloads 0
EVALUATION OF ANTI-OBESITY ACTIVITY OF SOLASODINE IN HIGH FAT DIET-INDUCED OBESITY IN RAT
Objective: This study was planned to study the anti-obesity activities of solasodine on high fat (HF) diet-induced obese rats.Methods: Wistar rats were divided into six groups. Control group (Group 1) received normal die...
PREVALENCE OF AMPC Î’-LACTAMASES IN CLINICAL ISOLATES OF E. COLI FROM A TERTIARY CARE RURAL HOSPITAL
Objective: Organisms over expressing AmpC (Ambler Class C) β-lactamases are of clinical concern because they restrict therapeutic options causing treatment failures and are increasing in occurrence worldwide. So the pre...
PHYCHEMISTRY, PHYTOCHEMICAL, PHARMACOLOGICAL AND MOLECULAR STUDY OF ZINGIBER OFFICINALE ROSCOE: A REVIEW
The rhizomes of Zingiber officinale Roscoe (Zingiberaceae), commonly known as ginger, is one of the most widely used spice and condiment. It is also an integral part of many traditional medicines and has been extensively...
UTILIZATION OF ETHNO-VETERINARY MEDICINAL PLANTS IN HASSAN DISTRICT OF KARNATAKA, INDIA
Objective: Present work deals with the studies on ethnoveterinary medicinal plants used by local people in Hassan district, Karnataka, India Methods: The survey was undertaken during 2015-2016 to collect the information...
COMPARISON OF ANGIOTENSIN RECEPTOR BLOCKER ALONE AND IN COMBINATION WITH VITAMIN D ANALOGUE IN REDUCING PROTEINURIA IN DIABETIC NEPHROPATHY PATIENTS
Objective: This study was designed to determine the synergistic effect of vitamin D analog added to angiotensin receptor blocker in reducing proteinuria in diabetic nephropathy patients.Methods: A randomized, open label,...