Comparative Analysis of Immunochromatographic Diagnostic Test and Microscopy for the Detection of Plasmodium Species
Journal Title: Journal of Indus Medical College - Year 2019, Vol 2, Issue 1
Abstract
INTRODUCTION: Light and thin film microscopy is a cost-effective gold standard for Malaria diagnosis is time consuming but needs expertise. In detecting Plasmodium species, immunochromatographic technique (ICT) has been claimed as an alternative to light microscopy. OBJECTIVE: This research was performed in reference to light microscopy of the smears to determine sensitivity and specificity of rapid malaria testing. PATIENTS AND METHODS: This study was carried out between January and september 2018 at Department of Pathology, Indus Medical College Hospital Tando Muhammad Khan. 105 patients with an evocative history of malaria were subjected to both tests, i.e. light microscopy and Immunochromatographic Technique (ICT) malaria P.f./Pan Rapid Test System for two methods comparison. RESULTS: The findings of the blood test showed that 49 patients (46.66%) were infected with malaria and the remaining 47 (44.76%) were negative for malaria. P.falciparum was found in 17 of positive patients. In the remaining 32 cases (65.30%) non-falciparum plasmodium species were found. The findings of the P.f./Pan ICT malaria test showed that 47 (44.76%) of the patient samples were positive for malaria parasites and 58 (55.23%) were negative for malaria parasites. 16 cases (32.65%) of P. falciparum infection and cases of non-falciparum plasmodium species accounted for remainder of the 31 cases ( 63.26%). ICT malaria therefore showed 94.25 per cent sensitivity and 95.00 per cent specificity for malaria parasite detection. CONCLUSION: Immunochromatographic technique offers user-friendly, accurate, sensitive alternative to slide microscopy for malaria diagnosis without adding cost and effort.
Authors and Affiliations
Shahzad Ali Jiskani, Saira Rajput, Umair Ali Soomro, Sikandar Ali Bhand
Keywords
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