Comparative analysis of research of acute toxicity of anthelmintic phytopreparation “Shchytnyk” by classical and alternative methods
Journal Title: Біологія тварин - Year 2017, Vol 19, Issue 1
Abstract
According to international requirements the testing of safety (toxicity) of various substances in living systems (human, animals and ecosystems) is carried out according to the harmonized methodological recommendations of the organization for Economic Cooperation and Development (OECD) and the International Conference on Harmonization of Technical Requirements for Registration of medicines (ICH). At the moment the scientific community and regulatory authorities of EU countries are paying great attention to the establishment of security and range of toxic effects of drugs, chemicals, biotechnology products and various nanomaterials. The article contains the results due to the comparative determination of parameters of acute toxicity of phytopreparation “Dryopteris filix-mas” using classical and alternative (European) methods. The principle of alternative (European) method for determining acute toxicity is based on a phased procedure using the minimum number of animals at each stage of the research, which suggests the classification of investigated substance of attributing it to one of the categories of toxicity classes, which are defined DL50 fixed threshold values. An alternative method for determining the acute toxicity class according to the OECD (test no. 423) does not apply mandatory a fatal outcome and for the experiment using the minimum number of animals. According to the classical method of determining acute toxicity was carried out in two stages: previous and extended. At the preliminary stage of research the drug was administered in doses of 500 to 4500 mg/kg, at intervals of 1000 mg/kg and each dose was used on 3 laboratory animals. In the expanded stage research groups of 6 animals in each are formed. The drug was injected in doses ranging from 5.000 to 13.000 mg/kg, at intervals of 2000 mg/kg of animals body weight. Class of toxicity was determined by JMA 85.2-37-736:2011. It was established that DL50 preparation “Dryopteris filix-mas” according to the classic method determining the parameters of acute toxicity is more than 13.000 mg/kg and the alternative — more than 5000 mg/kg. At a classic research it has been spent 39 laboratory animals, but at the alternative — 9 animals.
Authors and Affiliations
J. Perig
Keywords
Related Articles
- EP ID EP262634
- DOI 10.15407/animbiol19.01.083
- Views 147
- Downloads 0
The effect of silicon compounds on resistance of the rabbits
In the presented material, the results of the 14-day fertilization study and up to 20 days of lactation for the silicon citrate obtained by the method using nanotechnology, calculated from 50 μg Si/kg body weight and met...
Dynamics of phagocytic activity of neutrophils in rats under oxidative stress and the action of liposomal preparation
The article deals with the researches results of the developed complex liposomal preparation influence on dynamics of nonspecific level of natural resistance in rats under modeled oxidative stress caused with the use of...
Growth, development and reproductive function of female rats and their offspring viability at the conditions of the watering of different doses of citrate germanium
The article presents the results of studies of the effect of citrate germanium obtained using nanotechnology and method of chemical synthesis on growth, development and reproductive function of the body of female rats an...
Development of the immunosensor system for express-diagnosis of Pseudomonas aeruginosa
The article presents the results of development of the immune biosensor test systems for the express diagnostics of bacteria Pseudomonas aeruginosa, their detection in biological material and in the environment. Assessm...
Monitoring of microelements composition of shell eggs off african ostrich (Struthio camelus)
The shell eggs are the membrane that performs a number of vital functions in embryonic development of birds. To one of the important factors that affect the process of forming the shell belongs its content of trace elem...