Comparative Assessment of Extracts and Fractions of Dacryodesedulis Leaves and Stem barks From Two Locations as Sources of Antimicrobial Ingredients forSkin Care Products
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2018, Vol 13, Issue 5
Abstract
Plants are rich in a wide variety of phytochemicals with antimicrobial properties. Dacryodesedulis leaves and stem barks from two locations (Uyo and Ikom) were extracted with ethanol and water. The ethanol extracts were fractionated using solvent series of increasing polarity (dichloromethane, ethyl acetate, and nbutanol). Phytochemical screening of D. edulis leaves and stem barks extracts revealed the presence of phenols, flavonoids, tannins, saponins, cardiac glycosides, alkaloids, anthraquinones and terpenoids in some or all of the ethanol and aqueous extracts. The antimicrobial activity of the ethanol extracts, their respective fractions (dichloromethane, ethyl acetate, n-butanol and aqueous) and the aqueous extracts was determined using agar well diffusion technique. Ethanol extract of D. edulis leaves from Uyo inhibited all the micro-organisms (Staphylococcus aureus, Escherichia coli, Candida sp, Bacillus subtilis, Propionibacterium acnes and Pseudomonas aeroginosa) except Trichophyton sp., whereas ethyl acetate fraction of its counterpart from Ikom was active against all the pathogens. Also the ethyl acetate fractions of the stem barks from both locations inhibited more micro-organisms than the other fractions and extracts. The antimicrobial activity revealed by these extracts and fractions could be due to the secondary metabolites in the extract. This indicates the potential of D. edulis leaves and stem barks to serve as sources of antimicrobial ingredient for formulation of skin care products,to treat the underlying skin blemished caused by these micro-organisms. D. edulis leaves and stem barks from Ikom could be better antimicrobial ingredient for skin care products due to their lower minimum inhibitory concentration than its counterpart from Uyo.
Authors and Affiliations
Enengedi I. S. , Ekpa O. D. , Akpabio U. D.
Keywords
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- DOI 10.9790/3008-1305040817.
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