Comparative Studies on Methods of Tannase Assay
Journal Title: International Journal for Research in Applied Science and Engineering Technology (IJRASET) - Year 2015, Vol 3, Issue 3
Abstract
Five bacterial cultures were isolated from the tannery effluent and were screened by plate assay method. Three different methods mainly Colorimetric, UV spectrophotometric and Spectrophotometric methods were selected for the assay of tannase. These methods are selected on their mechanism and principle, feasibility, economical aspect and citations. Colorimetric method measures the liberated glucose; UV spectrophotometric method determines the esterase activity while Spectrophotometric method estimates the released gallic acid formed after the hydrolysis reaction, thus these methods estimate esterase as well as hydrolytic activity of enzyme. A comparative report was made between these methods and their characteristics and limitations were briefly mentioned. All the assays enabled estimation of tannase activity and have their own advantage and limitations. However, from the present study, modified spectrophotometric method is preferred due to its sensitivity and accuracy also it is easy to understand and perform but is expensive and requires preparation of exclusive solutions. In the present study, reports of these methods are presented so that it becomes easy to classify and select a method for tannase assay.
Authors and Affiliations
Dhruvil Brahmbhatt, H. A. Modi
Keywords
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