COMPARISON OF METHODS FOR MICROCYSTINS EXTRACTION OF WATER AND BIOMASS CYANOBACTERIAL SAMPLES
Journal Title: Българско списание за обществено здраве (Bulgarian Journal of Public Health) - Year 2016, Vol 0, Issue 3
Abstract
Eutrophication of freshwaters and appearance of cyanobacterial blooms connected with hepatotoxins microcystins, have become a worldwide problem. The maximal permissible level for the most dangerous microcystin-LR is 1 µg/dm3 for drinking and 20 µg/dm3 for bathing water. The aim of this survey is comparison of methods for extraction of microcystins from biomass and solid-phase extraction of microcystins from spiked water samples. The presented methods give a chance for reliable use of the easier and affordable method for extraction of microcystins from filtered cyanobacterial biomasses by a series of freezing and thawing and ultrasonic bath extraction. The experiment shows that the usage of cartridges and extraction disks for investigation of spiked water samples allows achieving a sufficient recovery of microcystins for analysis purposes (67-99 %, 79- 110 %, 74-85 % for cartridges and 87-93 % for disks respectively). Shorter extraction time per sample, a possibility for second and third usage of extraction disks and their commensurate price with that of cartridges makes them more appropriate for use. It is better to use extraction cartridges when a parallel extraction of a larger number of samples is necessary.
Authors and Affiliations
Vera Pavlova
Keywords
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