Comparison of Three Diagnostics Methods of Mycoplasma Gallisepticum in Batna Governorate
Journal Title: Journal of Veterinary Advances - Year 2013, Vol 3, Issue 3
Abstract
Mycoplasma gallisepticum infection is commonly designated as chronic respiratory disease in chickens. It is the most pathogenic avian Mycoplasma; however, strains may differ markedly in virulence. In this study, the technical performance of culture, a commercially available polymerase chain reaction (PCR) test and rapid plate agglutination (SPA) test were compared for the detection of Mycoplasma gallisepticum infections from 18 birds. Results showed a high percentage of positive samples of both culture and PCR tests (72.22% and 63.63% respectively). SPA showed a less positive rate (61.11%). the utilization of SPA towards MG diagnosis is limited by its reduced specificity and the high incidence of false positives Contradictory to other studies, bacteriology was more sensitive than PCR. Several studies support strongly the use of the PCR as a technique for the diagnosis of Mycoplasma infections since this technique is more sensitive than serology and culture. This study showed that it is not advisable to rely completely on one test (system) only.
Authors and Affiliations
H. Nouzha , A. Ammar , Bakir M. , K. L. Ahmed
Keywords
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