COMPARISON OF TYPHIDOT-EIA AND WIDAL TEST IN RESPECT TO POLYMERASE CHAIN REACTION AS DIAGNOSTIC PROCEDURES FOR EARLY DIAGNOSIS OF TYPHOID FEVER
Journal Title: Journal of Biomedical and Pharmaceutical Research - Year 2014, Vol 3, Issue 5
Abstract
Background: The public health burden of typhoid fever can be substantially reduced by early diagnosis and appropriate antibiotic therapy. Aim: This is a descriptive cross-sectional study; aimed to compare Typhidot-EIA test and Widal test in respect to polymerase chain reaction (PCR) for the detection of the early typhoid fever. Methods: A total of 80 suspected cases of typhoid fever attending Khartoum Hospitals between August and October 2013, were included in this study. Blood samples were collected from all cases during the first week of illness. Typhidot-EIA test, Widal test and PCR were performed for all samples. The Result: Out of 80 patients, 35 (43.8%) were found to have rising titer and considered as Widal positive patients and 21 (26.3%) were positive using Typhidot-EIA test; While only 10 (12.5%) were positive by PCR. The sensitivity and specificity of Widal test and Typhidot-EIA test against PCR were 60%, 59% and 80%, 81% respectively Conclusion: The DOT EIA IgM seems to be a practical alternative to Widal test for early diagnosis of typhoid fever
Authors and Affiliations
Hajir Abd-Alhafeez Abd-Alrahman| AL-Neelain University, Faculty of Medical Laboratory Science – Khartoum - Sudan, and Mohammed Nafi| AL-Neelain University, Faculty of Medical Laboratory Science – Khartoum - Sudan
Keywords
Related Articles
- EP ID EP3417
- DOI -
- Views 557
- Downloads 60
Evaluation of Microbial Contamination of Eye Make up Product: Mascara
Eyes are one of the most important features when it comes to beauty. Various cosmetics are used to enhance their level of attractiveness like mascaras, eyeliners eye shadow among many more. Out of these above products ma...
UTILIZATION PATTERN AND MEDICATION ADHERENCE OF ORAL ANTI-DIABETIC MEDICATIONS AMONG TYPE 2 DIABETES MELLITUS PATIENTS IN RURAL AREAS OF KARNATAKA, SOUTH INDIA.
Objective: To assess the utilization pattern and medication Adherence of oral anti-diabetic medications among patients with type 2 diabetes mellitus in rural areas of Bangalore East. Methodology: A prospective observatio...
A Review on Nanocochleate – A Novel Lipid Based Drug Delivery System.
Nanaocochleate represent a new approach for oral and systemic delivery of drugs . It is a novel lipid-based system which is suitable for the oral and systemic administration of a wide variety of molecules with important...
Effect of Orange (Citrus sinensis) Peel Oil on Lipid Peroxidation, Catalase activity and Hepatic Biomarker levels in Blood Plasma of Normo Rats
Dietary antioxidants are considered beneficial because of their potential protective role against oxidative stress, which is involved in the pathogenesis of multiple diseases such as cancer and coronary heart disease....
Review Article on Aspirin for prevention of cancer-A potential!
Cancer has been a worldwide threat now with millions of cases of morbidity and mortality owing to it. According to the World Health Organization (WHO), cancer accounted for 8.2 million deaths worldwide by 2012 and its pr...