CONSERVATION LEUKOCYTES IN THE CONDITIONS OF<br /> CRYOANABIOSIS (-40°С)
Journal Title: Журнал ÑтреÑÑ-физиологии и биохимии - Year 2006, Vol 2, Issue 1
Abstract
Trying to meet the requirements in leukocytes the clinicists are facing the pressing problem of conservation of cells in a functionally valid condition which is possible in deep cryoanabiosis (-196°C). This conservation method is not, for the number of reasons, available for the purpose of a wide use. The aim of the present was development of an efficient, readily available and economical method for conservation of leukocytes at a moderately low temperature of -40°C. It was shown that use of this method allows for conservation of blood nuclear cells in a functionally valid condition for a period of 30 days.
Authors and Affiliations
E Svedentsov, O Chtcheglova, T Tumanova
Keywords
Related Articles
- EP ID EP149681
- DOI -
- Views 98
- Downloads 0
Effect of Agrobacterium Induced Necrosis, Antibiotic Induced Phytotoxicity and Other Factors in Successful Plant Transformation
[i]Agrobacterium tumefaciens[/i] infection and antibiotic wash are the critical steps of [i]Agrobacterium[/i] mediated plant transformation procedure, most time responsible for lower transformation efficiency due to necr...
Antioxidative Defense Responses to lead-induced Oxidative Stress in Glycine max L. cv. Merrill grown in Different pH Gradient
Physiological and biochemical changes as well as the activities of anti-oxidative enzymes under lead (Pb[sup]2+[/sup]) phytotoxicity were investigated in 20 days old soybean (Glycine max L.) seedlings grown hydroponicall...
Fuelling of TCA cycle in hepatic cells Marwari goat during ambient temperature associated stress
The present study was launched to assess the effect of extreme ambient temperature associated stress on fuelling of TCA cycle in hepatic cells of Marwari goat. Based on the fact that whenever a hepatocyte needs fuel for...
Heat stress induced changes in metabolic regulators of donkeys from arid tracts in India
To find out heat stress induced changes in metabolic regulators of donkeys from arid tracts in India, blood samples were collected to harvest the serum during moderate and extreme hot ambiences. The metabolic enzymes det...
24-Epibrassinolide Mediated Changes in Photosynthetic pigments and Antioxidative Defence System of Radish Seedlings under Cadmium and Mercury Stress
The present work was conducted to study the effects of 24-EBL on photosynthetic pigments (total chlorophyll, chl a, chl b, carotenoid, anthocyanin and flavonoid content) and activities of antioxidative enzymes (guaiacol...