CRISPR-dCas9-mediated CpG island editing: A potential game-changer for diabetes treatment
Journal Title: Journal of Bioscience and Environment Research - Year 2025, Vol 2, Issue 1
Abstract
Type 2 diabetes mellitus (T2DM) is a chronic metabolic disorder characterized by hyperglycemia. Current therapeutic strategies primarily manage symptoms, leaving a substantial unmet need for curative interventions. This review explores the potential of CRISPR-dCas9-mediated CpG island editing as a promising therapeutic approach for T2DM. CpG islands, DNA regions enriched in cytosine-guanine dinucleotides, play a pivotal role in gene regulation. Their methylation status significantly influences gene expression. By targeting specific CpG islands within genes involved in glucose metabolism and insulin signaling, CRISPR-dCas9 can modulate gene expression and restore metabolic homeostasis. A particular focus is placed on the TXNIP gene, implicated in T2D pathogenesis. Reprogramming TXNIP expression using CRISPR-dCas9 offers potential therapeutic benefits, including protecting pancreatic beta cells, enhancing insulin sensitivity, and mitigating inflammation. While the potential of CRISPR-dCas9-mediated CpG island editing is clear and evident now, further steps are imperative to translate this approach into effective and safe therapies for T2DM patients.
Authors and Affiliations
Khokon Kumar Dutta
Keywords
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- EP ID EP765899
- DOI https://doi.org/10.69517/jber.2025.02.01.0003
- Views 11
- Downloads 0
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