CU (II) ACCUMULATION BY MARINE NEUTROPHIL SULFUR-OXIDIZING BACTERIA
Journal Title: Мікробіологія і біотехнологія - Year 2019, Vol 0, Issue 1
Abstract
Aim: to determine the ability of neutrophilic sulfur-oxidizing bacteria to extract Cu (II) ions from aqueous solutions. Methods. The object of the study was the ability of marine sulfur-oxidizing bacteria isolated from the water of the Black Sea near the Odessa Bay to extract copper from aqueous solution. To study the metalaccumulating activity of sulfur-oxidizing bacteria, we have selected strains that were resistant to copper at the level of 0.01–0.02 mol/l. The initial concentration of Cu (II) in aqueous solution was 1 mM. The period of cultivation of microorganisms in aqueous solution containing copper was 10 days. The residual content of Cu (II) in aqueous solution was determined by the atomic absorption method on AAS-1 devices (Germany) and C-115PC Selmi (Ukraine) at the wavelength of 324.7 nm for Cu. The reliability of the results obtained was assessed on the Student's criterion with probability of p <0.05. Results. It was established that sulfur-oxidizing bacteria isolated from the water of the Black Sea capable of accumulating heavy metals from aqueous solutions, and Cu-accumulating activity depends on the duration of the interaction between microorganisms and solution. The maximum level of extraction of Cu (II) from aqueous solution was equal 89.24% was recorded on the 10th day of cultivation using a strain of Thiobacillus sp. DKZ_4. On the seventh day of cultivation, the extraction of Cu (II) from aqueous solution with using a strain of Thiobacillus sp. DKZ_2 reached 85.25%. It was shown that the level of resistance to copper, which had been previously defined, did not affect the ability of bacteria to accumulate copper. Conclusions. Neutral sulfur-oxidizing bacteria isolated from the water of the Black Sea are capable of extracting from aqueous solution from 22.83% to 89.24% of Cu (II) and therefore they can be used to develop biosorption biotechnology especially in the field of water purification from heavy metals. The ability to accumulate Cu depends on the strain, but does not depend on MIC.
Authors and Affiliations
N. Vasylieva, L. Sliusarenko, T. V. Vasylieva
Keywords
Related Articles
- EP ID EP670053
- DOI 10.18524/2307-4663.2019.1(45).164171
- Views 101
- Downloads 0
THE PRODUCTIVITY OF MUTANT STRAIN STREPTOMYCES GLOBISPORUS 1912-4LCPHP7 IN THE DIFFERENT CONDITIONS
Aim. To determine the cause of decrease of carotenoid synthesis of lycopene in liquid medium from 4.2 to 2.8 mg/g of dry biomass in strains of Streptomyces globisporus 1912-4Lcp Hp7 and to select medium components and op...
MONITORING OF YEASTS CAUSED BAKERY PRODUCTS CONTAMINATION
Aim. The aim of the research was to determine the contaminants of bread, caused lime disease. Methods. Microbiological methods were used to determine the yeast contamination of bakery products with signs of bread’s disea...
EFFECT OF MULTIPROBIOTIC «SYMBITER ACIDOPHILIC» USED AGAINST THE BACKGROUND OF THE COURSE OF INJECTIONS OF CEFTRIAXONE ANTIBIOTIC ON PERITONEAL MACROPHAGE FUNCTIONAL ACTIVITY IN RAT
The aim of the work was to investigate the effect of probiotic preparation “Symbiter acidophilic” used against the background of the course of injection of ceftriaxone antibiotic on macrophage functional activity in Wist...
COMPARISON OF LONG-TERM EFFECT OF TWO DYSBIOSIS MODELS IN WISTAR RATS
Aim. To compare the changes of intestinal microbial composition of two models of dysbiosis induced by ceftriaxone (Cf) or the mix of ampicillin (Amp) and metronidazole (Met). Methods. The object of the study was the chan...
DESTRUCTION OF PHENOL AT THE FORMATION OF POLYVIDOUS BIOFILM ON NATURAL AND SYNTHETIC CARRIERS IN THE BIOFILTER
Aim. To determine the effectiveness of the process of water purification from phenol by microorganisms-destructors in the formation of a polyvidiofilm biofilm on natural and synthetic carriers in a biofilter. Methods. An...