CYTOPROTECTIVE EFFECT OF OCIMUM EXTRACT ON INJURED RENAL EPITHELIAL CELLS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 11
Abstract
Objectives: Renal cell injury is one of the important factors affecting urolithiasis. Crystals which aggregate to constitute the kidney stones are formed in the supersaturation condition in kidney. Reactive oxygen species generated due to the injury of renal epithelial cell by crystal formation and the injured cells itself participate in kidney stone formation. In the present study antioxidant, anti-inflammatory and cytoprotective effect of aqueous extract of Ocimum leaves were studied. Methods: Qualitative analysis of the plant extract was done using the basic phytochemical screening. DPPH (1, 1-diphenyl-2picrylhydrazyl) free radical scavenging activity test was done to estimate antioxidant activity of plant extract and protein denaturation method used to assess anti-inflammatory property of plant extract. The MTT (3-(4, 5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) assay was also done for the evaluation of cytoprotective effect of the plant extract on Vero cell line.Results: The results indicated that different concentrations of aqueous extract had strong antioxidant (94.5%±1.8) and anti-inflammatory (80.4%±1.3) activity. The aqueous extract increased the cell viability of Vero cell lines in a dose dependent manner. 40µg of the Ocimum extract exhibited 88.35%±1.32 cell viability of Vero cells which were injured with calcium oxalate crystals.Conclusion: Aqueous extract of Ocimum has cytoprotective effect on injured Vero cell lines. Anti-inflammatory and antioxidant property of Ocimum extract might be responsible for this cytoprotective nature of Ocimum extract.
Authors and Affiliations
Priyadarshini . , Kanika Jain
Keywords
Related Articles
- EP ID EP578208
- DOI -
- Views 82
- Downloads 0
FORMULATION AND EVALUATION OF MOISTURIZING CREAM CONTAINING SUNFLOWER WAX
Objective: The main objective of the present investigation was to design, prepare and evaluate moisturizing cream using sunflower wax.Methods: In the present work 32 full factorial design was applied to study the effect...
POPULATION PHARMACOKINETICS OF CONTINUOUS INFUSION OF FACTOR VIII IN HEMOPHILIA-A PATIENTS UNDERGOING ORTHOPEDIC SURGERY
Objective: to develop a population pharmacokinetic model taking into account blood losses during and after orthopedic surgery in adult hemophilia A patients receiving infusion of coagulation factor VIII, and to evaluate...
PRODUCTION OF THERAPEUTIC METHOTREXATE DEGRADING ENZYME AND STUDIES ON ITS NANOCOMPLEXES WITH HUMAN SERUM ALBUMIN
Objective: To improve the parent strain of Variovorax paradoxus for the production of methotrexate (MTX) degrading enzyme and to study the serum stability, release kinetics and functionality of the nano complexes of the...
HYDROPHOBIC INTERACTION OF 2-TRIFLUOROMETHYL-N10-SUBSTITUTED PHENOXAZINES WITH BOVINE SERUM ALBUMIN AND REVERSAL OF DRUG RESISTANCE IN BACTERIAL CELLS
Objective: The objective of this study was to report the hydrophobic interaction of 2-trifluoromethyl-N10-substituted phenoxazines with bovine serum albumin and reversal of drug resistance in bacterial cells.Methods: Bin...
COMPARATIVE PHYTOCHEMICAL EVALUATION OF ETHNOBOTANICALLY IMPORTANT MEDICINAL PARASITIC HERB: ALECTRA CHITRAKUTENSIS
Objective: Alectra chitrakutensis (M. A. Rau) R. Prasad & R. D. Dixit is a critically endangered and ethnobotanically very important plant. Official drug i.e. dried rhizome of the plant has been reported to be used f...