CYTOTOXICITY EVALUATIONS OF THE AQUEOUS EXTRACTS OF HERBAL MELANIN DRIVED FROM NIGELLA SATIVA SEEDS USING IN VIVO AND IN VITRO MODELS TESTS
Journal Title: World Journal of Pharmaceutical Research - Year 2017, Vol 6, Issue 12
Abstract
Background: Many studies over millennia have been carried out to determine a widely used medicinal plant throughout the world. Of these, most of the therapeutic properties of Nigella sativa (N. sativa) seeds are due to the presence of thymoquinone which is major bioactive component of the essential oil outcome, whereas melanin was unknown to exist in N. sativa before despite the fact that the seeds have a widely used medicinal plant throughout the world still need more studies. Objective: To investigate the cytotoxic effect of aqueous extracts of melanin extracted recently from N. sativa (NSM) seed coats on in vivo and in vitro safety outcome and thus used as a new bioactive contents and/or a promising natural therapeutic extract from a plant. Materials and Methods: Wistar rats and experimental mice in vivo tests with different NSM aqueous extracts doses according to their weight through intraperitoneal injection (i.p.) have been undertaken. Also, histopathological in vitro section tests using rat’s liver, intestines, kidney and testes segments were also observed and LD50 weas calculated. Either, different doses of NSM for in vitro viability of cells performed on Vero cells to analyses the cytotoxic activity of the extracts obtained. It was determined by cell death assay using the 3-(4,5-dimetylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) colorimetric assay tests. Also, morphological changes and CC50 were obtained and/or calculated. The cytotoxic effects of NSM extracts in experimental animals found safely, inducing action of NSM extracts in dose-response curves for each cell. Results: study showed that NSM extracts were significantly high safety during cell growth in a dose-dependent manner without inducing damage of in vivo and in-vitro cells used. The obtained data indicated the possible therapeutic and safety actions of the NSM aqueous extracts and that might pave the ways of its using as new bioactive compound and/or a promising health extract derived from a plant. Conclusion: The present work is an effort to provide in vivo and in vitro detailed and safety margins in using melanin extracts of the literature on scientific researches of the pharmacy-toxicological guide and thus lightening presence of melanin from N. sativa seeds.
Authors and Affiliations
Omar Abasher Al-Tayib
Keywords
Related Articles
- EP ID EP618298
- DOI -
- Views 186
- Downloads 0
HOLISTIC APPROACH OF AYURVEDA IN GERIATRICS: REVIEW ARTICLE
In present era medical science deals exclusively with the problem of ageing and diseases occur due to ageing. Geriatrics is emerging as a main challenging specialty because of ever growing population of aged people all o...
A VARIANT OF ANDROGRAPHIS OVATA C.B. CLARKE (ACANTHACEAE) - AN ENDEMIC MEDICINAL PLANT FROM INDIA
The aim of the present research was to investigate the identification of a variant ternate phyllotaxy of Andrographis ovata. This is the first announce on variant ternate phyllotaxy of Andrographis ovata Clarke.
SEROPREVALENCE AND RISK FACTORS OF TOXOPLASMA GONDII INFECTION AMONG HEALTHY BLOOD DONORS IN AL-RIBAT TEACHING HOSPITAL KHARTOUM STATE, SUDAN
Toxoplasmosis is an opportunistic, zoonotic disease with a worldwide distribution. There are large variations in the seroprevalence of T. gondii infection in different regions of the world. Toxoplasmosis is a common para...
ASSESSMENT THE EFFECT OF SINGLE NORMAL PREGNANCY ON EXPIRATORY FLOW RATES
Background: Dynamic PFTs are an important tool in the evaluation of the functional as well as fitness state of the respiratory system, also for the assessment of severity of illness. Objective: To assess the effect of si...
RETURN TO NATURE FOR EFFICACIOUS AND SAFER MEDICINAL PROSPECTS: A REVIEW
Traditional medicine also known as indigenous or folk medicine comprises medical knowledge systems that developed over generations within various societies before the era of modern medicine. We have gone through literatu...