Defoliation and Silverthiosulphate Pulse Treatment Prolongs the Vase Life of Cut Stalks of Clarkia amoena
Journal Title: Journal of Plant Science and Research - Year 2016, Vol 3, Issue 2
Abstract
A study was conducted to examine the vase life of cut stalks of Clarkia amoena. Stalks were harvested at 09:00 hrs, one day before anthesis of the first mature bud and held in following test solutions: Distilled water (Controls) and Sucrose (0.05 M, 0.10 M, 0.15 M and 0.20 M). Half of the stalks were manually defoliated before their transfer to these test solutions. Another set of stalks was pulse treated with either distilled water, Silverthiosulphate (STS) (0.5 mM) or Cycloheximide (CHI) (0.5 mM) for 1 hr at 25 °C and held in distilled water or Suc (0.1 M) + CoCl2 (0.1 mM). In distilled water (controls), vase life of stalks was 11.3 days. Manual defoliation of stalks held in distilled water enhanced vase life by 2-3 days. Sucrose in the holding solution had no significant effect on the vase life of either foliated or defoliated stalks. STS pulse treatment of stalks before transfer to distilled water significantly prolonged vase life by 3 days as compared to controls; whereas CHI pulse treatment reduced it by 7-8 days. Suc (0.1 M) + CoCl2 (0.1 mM) in the holding solution showed negative effect, irrespective of pulse treatment with either distilled water, STS or CHI. It is suggested that the cutoff of nutrient supply is not a limiting factor for the development and opening of buds on the cut stalks and that the ethylene and leaves are involved in the senescence of cut stalks of Clarkia amoena.
Authors and Affiliations
Zargar Mohmad Arief
Keywords
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