Designing primers potentially specific to Entamoeba gingivalis genes
Journal Title: Current Issues in Pharmacy and Medical Sciences - Year 2015, Vol 28, Issue 3
Abstract
Entamoeba gingivalis normally exists in the human oral cavity, namely in the gums, and brings about some specific diseases. However, it can also trigger some more serious illnesses. Among these are infections of the genital tract, acute osteomyelitis of the mandible and pulmonary abscess. Entamoeba gingivalis identification by light microscopy is difficult, hence polymerase chain reaction (PCR) is used. The contemporary primers for PCR are complement to 18S rRNA. This article informs the reader of the process that was involved in designing new primers for three genes which were thought to be present on the Entamoeba gingivalis genome, but their sequences were unknown. The newly obtained sequences of primers have better properties for identification purposes, compared to these which are currently used.
Authors and Affiliations
Jagoda Abramek
Keywords
Related Articles
- EP ID EP101286
- DOI 10.1515/cipms-2015-0064
- Views 88
- Downloads 0
Surgical treatment of dentoalveolar injury in the anterior region of maxilla
Traumatic injuries of teeth and alveolar bone are common in oral surgery departments. Maxillary incisors are the most frequently injured teeth. The right diagnosis is crucial for proper treatment method. Time elapsed bet...
The role of acetylcholine in drug addiction
Acetylcholine ACh, is involved in many CNS functions like sensory and motor processing, sleep, nociception, memory, mood, stress response, attention, arousal, motivation and reward. Due to the interaction with the dopami...
Adverse events (AE) costs assessed in Poland based on existing Drug Programs – methodology of the project
The Drug Programs, Pharmaceutical Care and Pharmaceutical Law section (DPPCPL) within Polish Pharmacoeconomical Society decided to perform a research on the impact of the adverse events treatment costs incurred by Nation...
Gastrointestinal hemorrhage in a 21-month-old girl in the course of lymphonodular hyperplasia (LNH). Case report
Gastrointestinal hemorrhage is an emergency condition requiring immediate diagnostics. The case of a 21-month-old girl is presented with massive bleeding from the lower gastrointestinal tract in the course of lymphonodu...
A comparison of the antioxidant properties of selected edible sprouts of the Legumes family
Germinating seeds of the Legumes family are a natural source of valuable nutrients. The aim of the study was to compare the antioxidant properties of selected edible sprouts from the Legumes family. Lentil and mung bean...