Detection of candidatus liberibacter asiaticus associated with citrus greening (huanglongbing) of mandarin by template preparation
Journal Title: Scientific Journal of Medical Science - Year 2013, Vol 2, Issue 2
Abstract
The polymerase ChainReaction (PCR) diagnosis is more reliable and sensitive diagnostic tool forgreening bacterium than other conventional approaches like Electron microscopy,DNA-DNA hybridization and immunofluorescence (IF) for detection of citrusgreening. During experiment, it was observed that sodium sulphite method of DNAisolation provided higher yield and better quality DNA than other methods.Primer C (450 bp) was more efficient in amplifying the DNA of greeningbacterium even at a very low concentration of 0.1 pg. To confirm thereliability of PCR, the greening bacterium was also detected ingraft-inoculated plants, which showed typical greening bacterium was alsodetected in graft-inoculated plants, which showed typical greening symptoms.Results showed amplification of 450 bp in PCR suggesting sampling in March ismore suitable for PCR detection of greening bacterium.
Authors and Affiliations
G. P. Jagtap| Department of Plant Pathology, College of Agriculture, M.K.V., Parbhani – 431 402 (MS)., M. V. Mahajan| Department of Plant Pathology, College of Agriculture, M.K.V., Parbhani – 431 402 (MS)., D. Utpal*| Department of Plant Pathology, College of Agriculture, M.K.V., Parbhani – 431 402 (MS).
Neck incision for comprehensive neck dissection
Over decades, a variety of incisions have been described for neck dissections. The current practice for neck dissection is to employ only a transverse incision along a suitable skin crease. We attempted a modification o...
Physiological and pharmaceutical properties of peppermint as a multipurpose and valuable medicinal plant
Peppermint is one of the oldest and most highly regarded herbs for appeasing digestion and may also restore digestive efficiency. In addition, peppermint has been known as disorders improving including: analgesic, ulcer,...
Shoulder pain
Shoulder pain is a common and important musculoskeletal problem. Compromised shoulder movement due to pain, stiffness, or weakness can cause substantial disability and affect a person’s ability to carry out daily activit...
Genetic mice models of Parkinson’s disease
The loss of dopaminergicneurons in the substancia nigra pars compacta leads to the characteristicsymptoms of Parkinson's disease (PD), whose Lewy bodies is a pathologic signalmost always found. Various monogenic forms ac...
Malignant solid tumors in basra pediatric oncology center
Malignant solid tumors represent about 50% of all malignant neoplasm in children aged less than 15 years ,They make more than 80% of all malignancies in infants. To focus on clinical and biological characteristics of...