Detection of cryptosporidium in fecal sample by three staining method from immunocompromised patients
Journal Title: IP International Journal of Medical Microbiology and Tropical Diseases - Year 2018, Vol 4, Issue 4
Abstract
Introduction Cryptosporidium species is the most common opportunistic enteric parasite encountered in the immunocompromised patientsObjective To evaluate Modified ZiehlNeelsen staining auramine phenol staining and safranine methylene blue for the diagnosis of intestinal cryptosporidiosisMaterials and Methods A total of 70 faecal samples from HIV seropositive were collected They were subjected for modified ZN stain safraninemethylene blue stain and auramine phenol stain for detection of cryptosporidiumResults Out of total 70 HIV patients 57 81 were positive for parasitic infection higher number of parasitic infection is caused by Cryptosporidia in 34 60 Of the 34 patients who had cryptosporidiosis were positive for auramine phenol technique 3397 of them were also positive with the modified ZN stain and 31910 were also positive with the safranine methylene blue stainConclusion Auramine phenol staining is a rapid sensitive and specific technique for diagnosis of intestinal cryptosporidiosisKeywords Cryptosporidium Modified ZN stain Safranine methylene blue stain Auramine phenol stain
Authors and Affiliations
Arti B. Ninama
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