Detection of Dengue Fever Virus Serotype – 4 by using One-Step Real-Time RT-PCR in Hodeidah, Yemen
Journal Title: Microbiology Research Journal International - Year 2016, Vol 14, Issue 6
Abstract
Background: Dengue and other fever like illnesses including chikungunya and malaria are common in Hodeidah, Yemen. Several outbreaks confirmed the presence of dengue serotypes 1-3. Aim: Confirm dengue fever infection and identify the circulating dengue virus serotypes in Hodeidah, using real time one step Reverse Transcription–Polymerase Chain Reaction (RT-PCR). Methods: Suspected dengue cases presented to health facilities between September 2012 and June 2013. Cases were informed about the study and asked to participate; 179 patients consented and were interviewed and blood samples were collected. The samples were tested at the National Centre of Public Health Laboratories (NCPHL) in Sana’a. Samples were initially tested by Enzyme Linkage Immunosorbent Assay (ELISA). Viral RNA was then extracted and prepared for serotypes detections using real time RT–PCR in one step pathway. Furthermore, agarose gel electrophoresis documentation system was used to confirm dengue serotypes. Results: Dengue virus was confirmed by RT-PCR in 69 of 179 specimens. The four dengue fever serotypes were identified. DENV-4 was the predominant serotype at 31.88%, followed by DENV-2 at 23.18%, DENV-3 at 20.28%, and DENV-1 at 10.14%. Concurrent infection with more than one serotype was detected in 14.49% of the specimens. Conclusion: We confirmed dengue virus infection using real time RT-PCR and identified DENV-4 serotype for the first time in Yemen. We also detected concurrent infections with more than on serotype. All serotypes are now present in Yemen increasing the risk of severe dengue and dengue hemorrhagic fever in future infections.
Authors and Affiliations
Murad Alahdal, Jamal Al-Shabi, Mahmoud Ogaili, Qais Yusuf Abdullah, Said Alghalibi, Aisha O. Jumaan, Mohammed Amood AL-Kamarany
Keywords
Related Articles
- EP ID EP350927
- DOI 10.9734/BMRJ/2016/24380
- Views 86
- Downloads 0
Prevalence of Enteropathogenic Escherichia coli (EPEC) in Adult Diarrhea Cases and their Antibiotic Susceptibility Pattern
Introduction: Diarrheagenic Escherichia coli (DEC), an important etiologic agent of diarrhea is a major public health problem in developing countries. Relatively few studies have reported the role of enteropathogenic E....
Co-infections; Their Role in HIV Acquisition and Disease Progression
HIV/AIDS disease manifestations play critical roles on the host's immune response to infections especially cell-mediated immunity which is central in combating many other infections, allowing opportunistic pathogens that...
The Effects of Different Storage Temperatures on the Microbial, Physicochemical and Organoleptic Quality Changes in the Shellfish "Ngolo" (Thais califera) from Nigeria
Aims: To investigate the effects of different storage temperatures (-15±2°C; 4±2°C and 29±2°C) on quality changes in ‘Ngolo’ (Thais califera) to evaluate the potential enhancement or otherwise of its domestic and global...
Genetic Characterization of Rhizobia Associated with Horse Gram [Macrotyloma uniflorum (Lam.) Verdc.] Based on RAPD and RFLP
Aim: To genetically characterize rhizobia collected from the root nodules of Horse gram plants grown in different soil samples by using the methods RAPD and RFLP. Place and Duration of Study: Root nodules were collected...
Profiling Rhizosphere Microbes on the Root of Maize (Zea mays) Planted in an Alfisol for Selection as Plant Growth Promoting Rhizobacteria (PGPR)
Maize (Zea mays L. Merill) root rhizosphere, being a metabolite-enriched niche was profiled with the objective of isolating Plant Growth Promoting Rhizobacteria (PGPR) for bio-fertilizer production. Isolation was carried...