DETECTION OF DNA DAMAGE IN OBESE CARDIOVASCULAR DISEASE PATIENTS AND COMPARISON BETWEEN GENDERS: A CASE-CONTROL STUDY
Journal Title: International Journal of Genetics - Year 2011, Vol 3, Issue 2
Abstract
Background- Obesity and cardiovascular disease (CVD) with the increased stress/ inflammation may cause DNA damage posing risk for malignancy hence assessment of DNA damage gains importance as a pre-cancerous biomarker. Methods-The alkaline Single Cell Gel Electrophoresis assay was carried out in peripheral blood leukocytes of obese-CVD patients (n=28; 14 males and 14 females) and matched controls (n=13; 7 males and 6 females). Multiple regression and analysis of variance were performed for effect of confounding/specific factors on DNA damage and the Student’s t-lest to compare differential values for factors associated with obesity-CVD and DNA damage. Results-In patients, genomic damage was significantly higher (p<0.05): damage frequency (59.21% in males, 60.14% in females), mean DNA migration length (37.53±4.74 μm, 19.16±2.60μm, respectively) in comparison to control data with significantly (p<0.01) increased damage in male patients. The attributes of obese-CVD patients and controls matched except for Body Mass Index (BMI), weight, waist circumference (WC) and waist hip ratio (WHR). Multivariate analysis of variance and multiple regression analysis revealed association of genetic damage with waist and hip circumferences in male patients. The t-test revealed that age, BMI, WHR, WC, cardiovascular disease sub-sets and drug usage had differential significance for genetic damage in female patients. Male patients with arrhythmia had significantly higher DNA damage as those on atenolol-rampril-furosemide therapy. Conclusion- The significantly increased DNA damage in obese-CVD patients may be from the nature of CVD, treatment duration, drug combinations and adiposity and gains importance as it imples a higher risk for age -related diseases and cancer.
Authors and Affiliations
G. GANDHI, J. KAUR, N. MAHAJAN
Keywords
Related Articles
- EP ID EP170159
- DOI 10.9735/0975-2862.3.2.66-71
- Views 105
- Downloads 0
REVIEW ARTICLE ON GENE THERAPY
Gene therapy can be broadly defined as the transfer of genetic material to cure a disease or at least to improve the clinical status of a patient. One of the basic concepts of gene therapy is to transform viruses into ge...
CONSERVATION MODELS ADOPTED FOR SHEEP AND GOAT IN INDIA
Domestication process occurred thousand years ago for sheep and goat genetic resources. In India, 42 breeds of sheep and 21 breeds of goat genetic resources is rich, diverse and locally adapted in its breeding tract. It...
RICE BREEDING LINES DEVELOPED WITH HIGHLY EFFICIENT SUBMERGENCE TOLERANCE THROUGH ADVANCED SINGLE SEED DESCENT METHOD FOR SEMI-LOWLAND AND -DEEP LOWLAND AREAS
In the present study, sixty-three breeding lines of rice cultivars adapted for semi-lowland and semi-deep lowland areas were developed for submergence tolerance through the single seed descent (SSD) method. In submergenc...
VARIATION IN THE YAK CALPASTATIN GENE (CAST)
Calpastatin (CAST) is a specific inhibitor of calpain (calcium-dependent cysteine protease). This study investigated the potential for variation in yak (Bos grennies) CAST. PCR-SSCP analysis of exon 6 of yak CAST reveale...
MALDI-TOF based Metabolomic approach
Metabolomics is the study of metabolite profiles in biological and pathological samples (urine, plasma, saliva, blood fat biopsies, plant cells, bacteria viruses and fungi. The metabolome is the most predictive phenotype...