Abstract

Objective: To determine the burden of gonorrhea among HIV patients using PCR technique. Methods: A cross sectional study was carried out in a HIV clinic of Sparsha Nepal from January to March, 2011. Standard microbiological procedures were followed during collection and processing of urine samples. DNA amplification, isolation and detection were carried out. Gonorrhoea diagnosis was done by multiplex polymerase chain reaction. Results: Among the 119 HIV positive patients, 12 (10.08%) were positive for N. gonorrheae. The cases were more among the males than in females. The age of patients ranged from 20- 45 years and the highest prevalence was among the age group 20-35 years. The distribution of gonorrhea infected patients according to marital status revealed that 8 cases were among the married patients. Conclusions: This study revealed that the incidence of gonorrhoea was higher in males and in married people. So, the control measures should be targeted to these groups of people.

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