DETECTION OF METHICILLIN RESISTANT STAPHYLOCOCCUS AREUS ISOLATED FROM NASAL CARRIAGE OF HEALTH CARE WORKERS BY POLYMERASE CHAIN REACTION
Journal Title: Journal of Experimental Biology and Agricultural Sciences - Year 2017, Vol 5, Issue 0
Abstract
Methicillin resistant Staphylococcus aureus (MRSA) and coagulase negative Staphylococci (MRCoNS) aremajor health problems worldwide. There is a growing concern about the rapid rise in resistance of nosocomialinfections to antimicrobial agents. The aim of the present study was to determine nasal colonization of MRSAand MRCoNS among healthcare workers (HCWs) in King Abdulaziz University hospital. A total of 100respondents were selected for this study and were classified into two groups: HCWs group which included 50technicians and nurses, and the control group which included 50 university students. Nasal swabs from anteriornares were cultured on selective media for the presence of mecA gene that is common to these bacteria.Polymerase chain reaction (PCR) and specific primers were used to determine mecA and coagulase gene (coa).The identified isolates were tested by restriction fragment length polymorphism (RFLP). Results of currentstudy revealed that among 50 HCWs, 21 (42%) were nasal carriers for MRCoNS and 12 (24%) for MRSA.The control group was negative for MRSA and MRCoNS. The results of the current study will help in makingdecision to prevent the spread of infections among workers in the field of health care.
Authors and Affiliations
Seham O. Alsulami, Huda A. Al Doghaither, Archana P. Iyer
Keywords
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- EP ID EP428022
- DOI 10.18006/2017.5(3).288.293
- Views 90
- Downloads 0
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